Comparison of the Toxicological Effects of Pesticides in Non-Tumorigenic MCF-12A and Tumorigenic MCF-7 Human Breast Cells
| Autor: | Lucia Coppola, Sabrina Tait, Enrica Fabbrizi, Monia Perugini, Cinzia La Rocca |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Estradiol
Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health endocrine disruptors herbicides idiopathic premature thelarche in vitro model mammary gland development MCF-12A MCF-7 nuclear receptors pesticides Adenosine Triphosphate Child Female Humans MCF-7 Cells Reactive Oxygen Species Breast Neoplasms Pesticides skin and connective tissue diseases |
| Zdroj: | International Journal of Environmental Research and Public Health; Volume 19; Issue 8; Pages: 4453 |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph19084453 |
| Popis: | Humans are exposed to residues of organophosphate and neonicotinoid pesticides, commonly used in agriculture. Children are particularly vulnerable and, among possible adverse outcomes, the increased incidence of premature mammary gland development (thelarche) has raised concern. We evaluated the toxicological effects of chlorpyrifos (CPF), imidacloprid (IMI) and glyphosate (GLY) at exposure concentrations occurring in children on the tumorigenic MCF-7 and non-tumorigenic MCF-12A breast cell lines, as representative of the target organ model, assessing cytotoxicity, apoptosis, necrosis, intracellular reactive oxygen species (ROS) and ATP levels, 17β-estradiol secretion and gene expression of nuclear receptors involved in mammary gland development. The pesticides decreased cell vitality in MCF-7 and cell proliferation in MCF-12A cells. ATP levels were decreased in MCF-7 cells by pesticides and apoptosis was increased in MCF-12A cells only by GLY (2.3 nM). ROS production was decreased by pesticides in both cell lines, except IMI (1.6 nM) in MCF-7 cells. Endocrine disrupting activity was highlighted by induction of 17β-estradiol secretion and modulation of the gene expression of estrogen alpha and beta, progesterone, androgen, and aryl hydrocarbon receptors in both cell lines. The use of MCF-7 and MCF-12A cells highlighted dissimilar modes of action of each pesticide at low human relevant concentrations. |
| Databáze: | OpenAIRE |
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