Plasma Circulating Tumor DNA Levels for the Monitoring of Melanoma Patients: Landscape of Available Technologies and Clinical Applications

Autor: Jean-Luc Coll, Joel Plumas, Benoit Busser, Amandine Hurbin, Julie Charles, Laurence Chaperot, Patrice Faure, Marie Therese Leccia, Julien Lupo, Stéphane Mouret, Pierre Hainaut, Lucie Sancey
Přispěvatelé: Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Biochemistry Pharmacology and Toxicology Department, Centre Hospitalo-Universitaire de Grenoble, Laboratoire de Virologie [CHU Grenoble], Centre Hospitalier Universitaire [Grenoble] (CHU), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Département de dermatologie, allergologie et photobiologie [Hôpital Michallon du CHU Grenoble Alpes], Centre Hospitalier Universitaire [Grenoble] (CHU)-Hôpital Michallon, Hypoxie et physiopathologies cardiovasculaire et respiratoire, Institut National de la Santé et de la Recherche Médicale (INSERM), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Hypoxie : Physiopathologie Respiratoire et Cardiovasculaire (HP2 ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Thomas, Frank
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Neuroblastoma RAS viral oncogene homolog
MESH: Neoplasm Proteins
Article Subject
[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
MESH: Melanoma
medicine.medical_treatment
lcsh:Medicine
MESH: DNA
Neoplasm
MESH: Monitoring
Physiologic
Review Article
Bioinformatics
Polymerase Chain Reaction
General Biochemistry
Genetics and Molecular Biology
Targeted therapy
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
Humans
Medicine
Digital polymerase chain reaction
Neoplasm Metastasis
Melanoma
Monitoring
Physiologic
MESH: Humans
General Immunology and Microbiology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
business.industry
lcsh:R
High mortality
MESH: Polymerase Chain Reaction
DNA
Neoplasm
General Medicine
medicine.disease
MESH: Neoplasm Metastasis
Neoplasm Proteins
3. Good health
Biomarker
030104 developmental biology
Circulating tumor DNA
030220 oncology & carcinogenesis
Cancer research
business
Zdroj: BioMed Research International
BioMed Research International, Hindawi Publishing Corporation, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩
BioMed Research International, 2017, 2017, pp.5986129. ⟨10.1155/2017/5986129⟩
BioMed Research International, Vol 2017 (2017)
ISSN: 2314-6133
2314-6141
DOI: 10.1155/2017/5986129⟩
Popis: Melanoma is a cutaneous cancer with an increasing worldwide prevalence and high mortality due to unresectable or metastatic stages. Mutations inBRAF,NRAS, orKITare present in more than 60% of melanoma cases, but a useful blood-based biomarker for the clinical monitoring of melanoma patients is still lacking. Thus, the analysis of circulating tumor cells (CTCs) and/or cell-free circulating tumor DNA (ctDNA) analysis from blood (liquid biopsies) appears to be a promising noninvasive, repeatable, and systemic sampling tool for detecting and monitoring melanoma. Here, we review the molecular biology-based strategies used for ctDNA quantification in melanoma patients, as well as their main clinical applications. Droplet digital PCR (ddPCR) and next generation sequencing (NGS) technologies appear to be two versatile and complementary strategies to study rare variant mutations for the detection and monitoring of melanoma progression. Among the different clinical uses of ctDNA, we highlight the assessment of molecular heterogeneity and the identification of genetic determinants for targeted therapy as well as the analysis of acquired resistance. Importantly, ctDNA quantification might also be a novel biomarker with a prognostic value for melanoma patients.
Databáze: OpenAIRE
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