Ultrasonography study of the skin wound healing process in gilthead seabream ( Sparus aurata )
| Autor: | María Ángeles Esteban, Diana Ceballos-Francisco, Nuria García-Carrillo, Alberto Cuesta |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Gilthead Seabream Skin wound Veterinary (miscellaneous) Aquatic Science Biology Neovascularization 03 medical and health sciences In vivo medicine Animals Skin Ultrasonography Skin repair Wound Healing integumentary system business.industry 04 agricultural and veterinary sciences Sea Bream 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Fish medicine.symptom business Wound healing |
| Zdroj: | Journal of Fish Diseases. 44:1091-1100 |
| ISSN: | 1365-2761 0140-7775 |
| DOI: | 10.1111/jfd.13370 |
| Popis: | This work aimed to carry out an in vivo study of the skin healing process in gilthead seabream (Sparus aurata) after being experimentally wounded. Firstly, the structure of normal skin was studied by real-time ultrasonography (Vevo Lab, VisualSonics) and light microscopy. Besides this, experimental wounds were made on the left flank of each fish with a circular biopsy punch (8 mm diameter) below the lateral line. The healing process was assessed on live fish at 0, 6, 11 and 23 days post-wounding using the real-time ultrasonography in B-mode and Power Doppler mode (Vevo 3100 FUJIFILM, VisualSonics). Through the ultrasonography images, both the skin structure and the evolution of the changes that wounds originated in the surrounding tissues were studied in vivo over time. Concomitantly, the pattern of neovascularization in the wounded area was followed during the healing process and it was demonstrated that, although the neovascularization started very early after the skin damage, it was increased in wounded areas from day 11 post-wounding onwards. The results obtained proved the utility and power of using ultrasounds in fish to evaluate in vivo complex biological processes in real time, which are difficult to study by other methodologies. The present data shed some light on the reparation of external injuries in aquatic vertebrates. |
| Databáze: | OpenAIRE |
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