Real‐timeex vivoperfusion of human lymph nodes invaded by cancer (REPLICANT): a feasibility study
| Autor: | Cristina Lombardelli, Kalnisha Naidoo, Richard Buus, Simi George, Natalie Woodman, Rachael Natrajan, Andrew Tutt, Julia Procter, Sarah E Pinder, Cheryl Gillett, Ashutosh Kothari, Tibor Kovacs, Erle Holgersen, Rachel Barrow-McGee, Syed Haider, Julie Owen, Kelvin Ramsey, Michael Douek, Peter A. Barry, Michael J. Shattock, Amy Gibson |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Axillary lymph nodes Breast Neoplasms Pathology and Forensic Medicine Metastasis immuno‐oncology 03 medical and health sciences breast cancer 0302 clinical medicine medicine Humans Original Paper lymph node metastasis business.industry Cancer normothermic perfusion Middle Aged medicine.disease Original Papers United Kingdom Perfusion 030104 developmental biology medicine.anatomical_structure Lymphatic Metastasis 030220 oncology & carcinogenesis Axilla Cancer cell Cancer research Feasibility Studies Immunohistochemistry Female Lymph Nodes Lymph business Ex vivo |
| Zdroj: | The Journal of Pathology |
| ISSN: | 1096-9896 0022-3417 |
| Popis: | Understanding how breast cancer (BC) grows in axillary lymph nodes (ALNs), and refining how therapies might halt that process, is clinically important. However, modelling the complex ALN microenvironment is difficult, and no human models exist at present. We harvested ALNs from ten BC patients, and perfused them at 37 °C ex vivo for up to 24 h. Controlled autologous testing showed that ALNs remain viable after 24 h of ex vivo perfusion: haematoxylin and eosin‐stained histological appearance and proliferation (by Ki67 immunohistochemistry) did not change significantly over time for any perfused ALN compared with a control from time‐point zero. Furthermore, targeted gene expression analysis (NanoString PanCancer IO360 panel) showed that only 21/750 genes were differentially expressed between control and perfused ALNs (|log2 FC| > 1 and q |
| Databáze: | OpenAIRE |
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