Diabetes induces macrophage dysfunction through cytoplasmic dsDNA/AIM2 associated pyroptosis
Autor: | Qianming Chen, Pengfei Zhao, Peng Zhang, Qi Wang, Ning Ji, Lulingxiao Nie, Ziqi Yue |
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Rok vydání: | 2021 |
Předmět: |
Periodontium
0301 basic medicine Aging Cytoplasm Immunology Biology Models Biological Diabetes Mellitus Experimental Proinflammatory cytokine Pathogenesis Mice 03 medical and health sciences 0302 clinical medicine Immune system Phagocytosis Diabetes mellitus Pyroptosis medicine Animals Immunology and Allergy Macrophage Antigen Presentation Innate immune system Chemotaxis Macrophages DNA Cell Biology medicine.disease Metformin DNA-Binding Proteins Mice Inbred C57BL Glucose RAW 264.7 Cells 030104 developmental biology Hyperglycemia 030220 oncology & carcinogenesis Cytokines Inflammation Mediators medicine.drug |
Zdroj: | Journal of Leukocyte Biology. 110:497-510 |
ISSN: | 1938-3673 0741-5400 |
Popis: | Diabetes is emerging as a severe global health problem that threatens health and increases socioeconomic burden. Periodontal impairment is one of its well-recognized complications. The destruction of the periodontal defense barrier makes it easier for periodontal pathogens to invade in, triggering a greater inflammatory response, and causing secondary impairment. Macrophages are the major immune cells in periodontium, forming the frontier line of local innate immune barrier. Here, we explored the periodontal impairments and functional changes of macrophages under the diabetic and aging conditions. Besides, we further explored the molecular mechanism of how hyperglycemia and aging contribute to this pathogenesis. To test this, we used young and aged mice to build diabetic mice, and metformin treatment was applied to a group of them. We demonstrated that under hyperglycemia conditions, macrophage functions, such as inflammatory cytokines secretion, phagocytosis, chemotaxis, and immune response, were disturbed. Simultaneously, this condition elevated the local senescent cell burden and induced secretion of senescence-associated secretory phenotype. Meanwhile, we found that expressions of Gasdermin D (GSDMD) and caspase-1 were up-regulated in diabetic conditions, suggesting that the local senescent burden and systemic proinflammatory state during diabetes were accompanied by the initiation of pyroptosis. Furthermore, we found that the changes in aged condition were similar to those in diabetes, suggesting a hyperglycemia-induced pre-aging state. In addition, we show that metformin treatment alleviated and remarkably reversed these functional abnormalities. Our data demonstrated that diabetes initiated macrophage pyroptosis, which further triggered macrophage function impairments and gingival destructions. This pathogenesis could be reversed by metformin. |
Databáze: | OpenAIRE |
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