The pseudoenzyme PDX1.2 boosts vitamin B6 biosynthesis under heat and oxidative stress in Arabidopsis
| Autor: | Pedro Surriabre, Svetlana Boycheva, Markus Kaufmann, Marina Tambasco-Studart, Teresa B. Fitzpatrick, Cyril Moccand, Maja Raschke |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Hot Temperature endocrine system diseases Nitrogenous Group Transferases Regulator Arabidopsis Arabidopsis/genetics/physiology medicine.disease_cause Biochemistry Antioxidants Biosynthesis Enzyme Catalysis Plant Biochemistry Pyridoxal Phosphate Stress Response chemistry.chemical_compound Models Pyridoxal phosphate Gene knockdown biology food and beverages ddc:580 Gene Knockdown Techniques Vitamin endocrine system Physiological Molecular Sequence Data Vitamin B 6/metabolism Nitrogenous Group Transferases/chemistry/genetics/metabolism Stress digestive system Cofactor Stress Physiological Carbon-Nitrogen Lyases medicine Amino Acid Sequence Molecular Biology Arabidopsis Proteins/chemistry/genetics/metabolism Arabidopsis Proteins Molecular Cell Biology biology.organism_classification Vitamin B 6 Oxidative Stress chemistry biology.protein Enzymology Oxidative stress |
| Zdroj: | Journal of Biological Chemistry, Vol. 289, No 12 (2014) pp. 8203-8216 Journal of Biological Chemistry, 289 (12) |
| ISSN: | 1083-351X 0021-9258 |
| Popis: | Vitamin B6 is an indispensable compound for survival, well known as a cofactor for numerous central metabolic enzymes and more recently for playing a role in several stress responses, particularly in association with oxidative stress. Regulatory aspects for the use of the vitamin in these roles are not known. Here we show that certain plants carry a pseudoenzyme (PDX1.2), which is involved in regulating vitamin B6 biosynthesis de novo under stress conditions. Specifically, we demonstrate that Arabidopsis PDX1.2 enhances the activity of its catalytic paralogs by forming a heterododecameric complex. PDX1.2 is strongly induced by heat as well as singlet oxygen stress, concomitant with an enhancement of vitamin B6 production. Analysis of pdx1.2 knockdown lines demonstrates that boosting vitamin B6 content is dependent on PDX1.2, revealing that this pseudoenzyme acts as a positive regulator of vitamin B6 biosynthesis during such stress conditions in plants. Journal of Biological Chemistry, 289 (12) ISSN:0021-9258 ISSN:1083-351X |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|