Adenovirus Messenger RNA in Mammalian Cells: Failure of Polyribosome Association in the Absence of Nuclear Cleavage
| Autor: | C. Swart, P. M. McGuire, L. D. Hodge |
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| Rok vydání: | 1972 |
| Předmět: |
Five-prime cap
viruses RNA-dependent RNA polymerase Biology Nucleic Acid Denaturation Adenoviridae Centrifugation Density Gradient Pyrroles RNA Messenger Biological Sciences: Cell Biology Cell Nucleus Antibiotics Antineoplastic Cyanides Multidisciplinary Intron Nucleic Acid Hybridization RNA Nucleosides Molecular biology Post-transcriptional modification Molecular Weight Kinetics RNA silencing Pyrimidines Genetic Code RNA editing RNA Viral Ribosomes Small nuclear RNA HeLa Cells |
| Zdroj: | Proceedings of the National Academy of Sciences. 69:1578-1582 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.69.6.1578 |
| Popis: | The nuclear synthesis of adenovirus-specific RNA late in the infectious cycle in the presence of toyocamycin (an adenosine analogue) has been investigated. There is reduced synthesis of viral RNA with an accumulation of virus-specific RNA in the molecular weight range of at least 4 to 8 × 10 6 . No new viral RNA associates with cytoplasmic polyribosomes. In addition, hybridization competition experiments indicate a 70% competition between these large nuclear transcripts and polyribosome-associated viral RNA that was synthesized in the absence of inhibitor. These data are consistent with the following interpretations: complete nuclear processing of viral RNA is necessary for polyribosome association, and precursor viral message(s) contain sequences that are lost normally during post-transcriptional processing. |
| Databáze: | OpenAIRE |
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