Production of Hydroxlated Flavonoids with Cytochrome P450 BM3 Variant F87V and Their Antioxidative Activities

Autor:	Chihiro Ota, Emi Kitamura, Norihiko Misawa, Chiemi Maeda, Yoko Aoki, Toshihiko Otomatsu, Kazutoshi Shindo
Rok vydání:	2013
Předmět:	Chalcone Bioconversion Stereochemistry Recombinant Fusion Proteins Flavonoid medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Antioxidants Mixed Function Oxygenases Substrate Specificity Analytical Chemistry Structure-Activity Relationship chemistry.chemical_compound Bacterial Proteins Cytochrome P-450 Enzyme System Escherichia coli medicine Molecular Biology Gene NADPH-Ferrihemoprotein Reductase Flavonoids chemistry.chemical_classification Recombinant escherichia coli biology Organic Chemistry Cytochrome P450 General Medicine Monooxygenase chemistry biology.protein Oxidation-Reduction NADP Biotechnology
Zdroj:	Bioscience, Biotechnology, and Biochemistry. 77:1340-1343
ISSN:	1347-6947 0916-8451
Popis:	A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9010d666c6884a5be44aa3fbbd085db5 https://doi.org/10.1271/bbb.130148 Zobrazit plný text záznamu