Dissipation of DvSnf7 RNA from Late‐Season Maize Tissue in Aquatic Microcosms
| Autor: | Joshua R. Fischer, Glennon J. Rogan, Zihong Song, Marianne Malven, Gillian R. MacQuarrie |
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| Rok vydání: | 2020 |
| Předmět: |
Crops
Agricultural 0106 biological sciences Aquatic Organisms Geologic Sediments Time Factors Health Toxicology and Mutagenesis Zoology Genetically modified crops 010501 environmental sciences Biology Risk Assessment Zea mays 01 natural sciences Water column Limit of Detection Animals Environmental Chemistry 0105 earth and related environmental sciences Invertebrate Genetically modified maize Aquatic ecosystem Water RNA Plants Genetically Modified 010602 entomology SmartStax RNA Plant Seasons Microcosm |
| Zdroj: | Environmental Toxicology and Chemistry. 39:1032-1040 |
| ISSN: | 1552-8618 0730-7268 |
| Popis: | The commercialization of RNA-based agricultural products requires robust ecological risk assessments. Ecological risk is operationally defined as a function of exposure and adverse effects. Information on the environmental fate of RNA-based plant-incorporated protectants is essential to define routes and duration of exposure to potentially sensitive nontarget organisms. Providing these details in problem formulation helps focus the ecological risk assessment on the relevant species of concern. Postharvest plant residue is often considered to be the most significant route of exposure for genetically modified crops to adjacent aquatic environments. Previous studies have shown that DvSnf7 RNA from SmartStax PRO maize dissipates rapidly in both terrestrial and aquatic environments. Although these studies suggest that direct exposure to DvSnf7 RNA is likely to be low, little is known regarding the fate of DvSnf7 RNA produced in plants after entering an aquatic environment. This exposure scenario is relevant to detritivorous aquatic invertebrates that process conditioned maize tissues that enter aquatic environments. To assess potential exposure to shredders, dissipation of DvSnf7 RNA expressed maize tissue was evaluated following immersion in microcosms containing sediment and water. Concentrations of DvSnf7 RNA in the tissue were measured over a duration of 21 d. The DvSnf7 RNA dissipated rapidly from immersed maize tissue and was undetectable in the tissues after 3 d. Concentrations of DvSnf7 RNA found in tissue as well as calculated water column concentrations were below levels known to elicit effects in a highly sensitive surrogate species, supporting the conclusion of minimal risk to aquatic nontarget organisms. Environ Toxicol Chem 2020;39:1032-1040. © 2020 SETAC. |
| Databáze: | OpenAIRE |
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