Active β-Catenin Signaling in the Small Intestine of Humans During Infancy
| Autor: | Zenab M. Dudhwala, Paul A. Drew, David J. Moore, Adrian G. Cummins, Gordon S. Howarth |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Paneth Cells medicine.medical_specialty Duodenum Physiology Crypt Columnar Cell Biology digestive system Proto-Oncogene Proteins c-myc Andrology 03 medical and health sciences 0302 clinical medicine Axin Protein Internal medicine medicine Humans Intestinal Mucosa Wnt Signaling Pathway beta Catenin Aged Stem Cells Age Factors Gastroenterology Wnt signaling pathway Infant Middle Aged Hepatology Small intestine medicine.anatomical_structure Cytoplasm 030220 oncology & carcinogenesis Female 030211 gastroenterology & hepatology Stem cell Homeostasis |
| Zdroj: | Digestive Diseases and Sciences. 64:76-83 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-018-5286-y |
| Popis: | Wnt-β-catenin signaling is essential for homeostasis of intestinal stem cells in mice and is thought to promote intestinal crypt fission. The aim of this study was to investigate Wnt-β-catenin signaling in intestinal crypts of human infants. Duodenal biopsies from nine infants (mean, range 0.9 years, 0.3–2 years) and 11 adults (mean, range 43 years, 34–71 years) were collected endoscopically. Active β-catenin signaling was assessed by cytoplasmic and nuclear β-catenin, nuclear c-Myc, and cytoplasmic Axin-2 expression in the base of crypts. Tissues were stained by an immunoperoxidase staining technique and quantified as pixel energy using cumulative signal analysis. Data were expressed as mean ± SD and significance assessed by Student’s t test. Crypt fission was significantly higher in infants compared to adults (16 ± 8.6% versus 0.7 ± 0.6%, respectively, p |
| Databáze: | OpenAIRE |
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