FUNCTIONAL INTERCHANGEABILITY OF DNA REPLICATION GENES IN SALMONELLA TYPHIMURIUM AND ESCHERICHIA COLI DEMONSTRATED BY A GENERAL COMPLEMENTATION PROCEDURE
| Autor: | Barbara C. Osmond, Russell Maurer, Eugene Shekhtman, David Botstein, Annette Wong |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA Replication
Salmonella typhimurium Salmonella Genotype Viral Plaque Assay Investigations Biology medicine.disease_cause Transduction Genetic Protein-fragment complementation assay Escherichia coli Genetics medicine Genomic library Gene Alleles Genetic Complementation Test DNA replication Bacteriophage lambda Molecular biology DnaA Complementation Genes Genes Bacterial Mutation |
| Zdroj: | Genetics. 108:1-23 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/108.1.1 |
| Popis: | Twenty-four genes from Salmonella typhimurium that affect DNA replication were isolated from a λ-Salmonella genomic library by lysogenic complementation of temperature-sensitive mutants of Salmonella or E. coli, using a new plaque complementation assay. The complementing λ clones, which make red plaques in this assay, and noncomplementing mutant derivatives, which make uncolored plaques, were used to further characterize the temperature-sensitive Salmonella mutants and to establish the functional similarity of E. coli and Salmonella DNA replication genes. For 17 of 18 E. coli mutants representing distinct loci, a Salmonella gene that complemented the mutant was found. This result indicates that single Salmonella replication proteins are able to function in otherwise all E. coli replication complexes and suggests that the detailed properties of Salmonella and E. coli replication proteins are very similar. The other seven Salmonella genes that were cloned were unrelated functionally to any E. coli genes examined. —As an aid to the derivation of chromosomal mutations affecting some of the cloned genes, a general method was developed for placing a transposon in the Salmonella chromosome in a segment corresponding to cloned DNA. Chromosomal mutations were derived in Salmonella affecting a gene (dnaA) that was cloned by complementation of an E. coli mutant by using the transposon-encoded drug resistance as a selectable marker in local mutagenesis. |
| Databáze: | OpenAIRE |
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