Sixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease
| Autor: | Chantal Faure, Sébastien Massart, Cécile Robin, Sergio Murolo, François Maclot, Gianfranco Romanazzi, Michela Chiumenti, Yoann Brans, Thierry Candresse, Marie Lefebvre, Teresa Barreneche, Angelantonio Minafra, Rémy J. Petit, Clément Larue, Armelle Marais |
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| Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università Politecnica delle Marche [Ancona] (UNIVPM), CTIFL PRIGONRIEUX FRA, Partenaires IRSTEA, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Biodiversité, Gènes & Communautés (BioGeCo), Invenio, Unité de recherche TERRA [Gembloux], Gembloux Agro-Bio Tech [Gembloux], Université de Liège-Université de Liège, Institute for Sustainable Plant Protection of National Research Council (ISPP - CNR) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pathogen detection etiology [SDV]Life Sciences [q-bio] Caulimoviridae Mosaic (geodemography) Genome Viral Plant Science Disease Biology Fagaceae 01 natural sciences DNA sequencing Open Reading Frames 03 medical and health sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology High throughput sequencing Badnavirus Phylogeny Plant Diseases Disease description virus phytopathogène biology.organism_classification viral disease Virology [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy 3. Good health 030104 developmental biology Castanea sp Viral disease virologie végétale Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Phytopathology Phytopathology, American Phytopathological Society, 2021, 111 (6), pp.1051-1058. ⟨10.1094/PHYTO-09-20-0420-R⟩ |
| ISSN: | 0031-949X |
| DOI: | 10.1094/PHYTO-09-20-0420-R⟩ |
| Popis: | International audience; Although chestnut mosaic disease (ChMD) was described several decades ago, its etiology is still not clear. Using classical approaches and high-throughput sequencing (HTS) techniques, we identified a novel Badnavirus that is a strong etiological candidate for ChMD. Two disease sources from Italy and France were submitted to HTS-based viral indexing. Total RNAs were extracted, ribodepleted, and sequenced on an Illumina NextSeq500 (2 × 150 nt or 2 × 75 nt). In each source, we identified a single contig of ≈7.2 kb that corresponds to a complete circular viral genome and shares homologies with various badnaviruses. The genomes of the two isolates have an average nucleotide identity of 90.5%, with a typical badnaviral genome organization comprising three open reading frames. Phylogenetic analyses and sequence comparisons showed that this virus is a novel species; we propose the name Chestnut mosaic virus (ChMV). Using a newly developed molecular detection test, we systematically detected the virus in symptomatic graft-inoculated indicator plants (chestnut and American oak) as well in chestnut trees presenting typical ChMD symptoms in the field (100 and 87% in France and Italy surveys, respectively). Datamining of publicly available chestnut sequence read archive transcriptomic data allowed the reconstruction of two additional complete ChMV genomes from two Castanea mollissima sources from the United States as well as ChMV detection in C. dentata from the United States. Preliminary epidemiological studies performed in France and central eastern Italy showed that ChMV has a high incidence in some commercial orchards and low within-orchard genetic diversity. |
| Databáze: | OpenAIRE |
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