CD133 Expression in Normal Skin and in Epithelial Cutaneous Tumors
| Autor: | J. M. Azaña, Angel G. Martin, R. Calero-Oliver, R. Serrano-Vargas, S. H. Nam-Cha, E. Poblet, E. Escario |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Skin Neoplasms Article Subject Cellular differentiation Primary Cell Culture lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology Glandular Differentiation Flow cytometry Antigens CD Cancer stem cell Cell Line Tumor Biomarkers Tumor medicine Carcinoma Humans AC133 Antigen Neoplasms Glandular and Epithelial Aged Glycoproteins Aged 80 and over General Immunology and Microbiology medicine.diagnostic_test lcsh:R Apocrine Cell Differentiation General Medicine Middle Aged Flow Cytometry medicine.disease Gene Expression Regulation Neoplastic carbohydrates (lipids) embryonic structures Cancer cell Carcinoma Squamous Cell Neoplastic Stem Cells Immunohistochemistry Female Peptides Research Article |
| Zdroj: | BioMed Research International, Vol 2013 (2013) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2013/385604 |
| Popis: | Background. Expression of human CD133 (human prominin-1) in cancer cells has been postulated to be a marker of stemness and is considered as a putative marker of cancer stem cells (CSCs). We designed a study to describe the expression pattern of CD133 in normal skin and in epithelial cutaneous neoplasms.Methods. The CD133 immunohistochemical expression of forty-three eccrine and apocrine tumors was compared to that observed in other epithelial tumors of the skin. In addition, flow cytometry was used to detect the CD133 expression of four epithelial skin neoplasms, including one porocarcinoma.Results. CD133 immunoreactivity at the apical or at the apicolateral surface of cells forming glandular structures was observed. Cells from solid areas of benign or malignant tumors were not stained. The porocarcinoma derived culture cells showed a 22% of CD133 positive cells using flow cytometry, while squamous cell carcinoma cultures contained less than 0.1%.Conclusions. These observations indicate that CD133 is a specific marker of glandular differentiation that could be included in the diagnostic panel of cutaneous tumors with possible eccrine or apocrine differentiation. However, the use of CD133 expression as a marker of CSCs should be interpreted with caution in experiments of skin. |
| Databáze: | OpenAIRE |
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