EBV-inducing factor from platelets exhibits growth-promoting activity for NIH 3T3 cells
| Autor: | C H Heldin, P Höfler, U Birnbaum, G Bauer |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Blood Platelets
Herpesvirus 4 Human Platelet-derived growth factor medicine.medical_treatment Mice Inbred Strains Biology Antibodies General Biochemistry Genetics and Molecular Biology 3T3 cells Cell Line Mice chemistry.chemical_compound medicine Animals Humans Platelet Growth Substances Molecular Biology Cells Cultured Platelet-Derived Growth Factor General Immunology and Microbiology General Neuroscience Growth factor food and beverages Biological activity Burkitt Lymphoma Molecular biology Rats Kinetics medicine.anatomical_structure Isoelectric point chemistry Biochemistry Cell culture biology.protein Cell Division Platelet-derived growth factor receptor Research Article |
| Zdroj: | The EMBO Journal. 4:1957-1961 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1985.tb03877.x |
| Popis: | An Epstein-Barr virus-indicating factor (EIF) has been purified from serum and platelets. We show here that highly purified preparations of platelet EIF exhibit growth-promoting activity for NIH 3T3 cells maintained in platelet-poor plasma. The Epstein-Barr virus (EBV)-inducing activity and growth-promoting activity co-elute upon gel chromatography under non-dissociating as well as dissociating conditions and co-migrate in SDS-gel electrophoresis, supporting the notion that both activities reside on the same molecule. Furthermore, both activities require a pH shock for full activity and act in the same concentration range. The growth-promoting activity of EIF can be differentiated from that of platelet-derived growth factor (PDGF), biologically (on the basis of differential response of cell lines to both factors), biochemically (on the basis of differences in isoelectric points and mol. wts. and the requirement of EIF to become activated by a pH shock) and by the lack of inhibition of EIF by antibody to PDGF. |
| Databáze: | OpenAIRE |
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