Effect of different mini-volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer-assisted semen analyzer
| Autor: | Hugo Hector Ortega, James L. Chitwood, Pablo J. Ross, Cristóbal Fresno, Luis B. Ferré, M. E. Kjelland |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
endocrine system TWO-LAYER CENTRIFUGATION Serial dilution SPERM SELECTION medicine.medical_treatment Centrifugation Semen SEX-SORTED SPERM Biology Andrology 03 medical and health sciences Colloid 0302 clinical medicine Endocrinology Image Processing Computer-Assisted medicine Animals Colloids Sex Preselection Sperm motility Cryopreservation 030219 obstetrics & reproductive medicine Chromatography In vitro fertilisation SPERM RECOVERY urogenital system Ciencias Veterinarias 0402 animal and dairy science 04 agricultural and veterinary sciences Flow Cytometry Spermatozoa 040201 dairy & animal science Sperm Dilution Semen Analysis SPERM VIABILITY CIENCIAS AGRÍCOLAS Cattle Animal Science and Zoology SINGLE-LAYER CENTRIFUGATION Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 53:26-33 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.13048 |
| Popis: | Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 106 sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex-sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane-coated silica colloid dilutions and layering configurations during centrifugation of sex-sorted sperm was examined over an extended period of incubation time. Sperm recovery and viability after centrifugation using the colloid separation technique were measured along with several sperm motility parameters using CASA. For this purpose, frozen and thawed sex-sorted sperm samples were centrifuged using mini-volume single-layer (40%, 60% and 80%) and mini-volume two-layer (45%/90%, 40%/80% and 30%/60%) separation configurations using PureSperm®. A single layer of 40% PureSperm® recovered significantly more sex-sorted sperm (78.07% ± 2.28%) followed by a single layer of 80% PureSperm® (68.43% ± 2.33%). The lowest sperm recovery was obtained using a two-layer PureSperm® dilution of 45%/90% (47.57% ± 2.33%). Single-layer centrifugation recovered more sorted sperm (68.67% ± 1.74%) than two layer (53.74% ± 1.74%) (p |
| Databáze: | OpenAIRE |
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