An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury
| Autor: | Fung-Jou Lu, I-Jen Chen, Yang-Hwei Tsuang, Jui-Sheng Sun, Wan-Ching Huang, Yi-Shiong Hang |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Kidney Critical Care and Intensive Care Medicine medicine.disease_cause Luminol law.invention chemistry.chemical_compound law Internal medicine medicine Animals Muscle Skeletal Lung Whole blood Chemiluminescence Thermal injury Superoxide business.industry Myocardium Kidney metabolism Free Radical Scavengers General Medicine Disease Models Animal Oxidative Stress Endocrinology Liver chemistry Anesthesia Acute Disease Luminescent Measurements Emergency Medicine Acridines Indicators and Reagents Surgery Rabbits Burns business Oxidative stress |
| Zdroj: | Burns. 24:225-231 |
| ISSN: | 0305-4179 |
| DOI: | 10.1016/s0305-4179(97)00115-0 |
| Popis: | It is not easy to detect oxygen free radicals directly because of their very short half-life. In the present study, a sensitive ultra-weak chemiluminescence detector was used to detect the generation of oxygen free radicals following thermal injury. Twelve New Zealand white rabbits were used in this study. After anesthesia, the bilateral hind-limbs were exposed to 100 degrees C water for 30 s. Six control animals were exposed to 22 degrees C water to act as a control. The chemiluminescence of whole blood and visceral organs were measured with both luminol-amplified t-butyl hydroperoxide-initiated and lucigenin-initiated methods. The results showed that chemiluminescence of blood was affected significantly by acute thermal injury. The chemiluminescence of blood increased significantly at 1 h following acute thermal injury, reached a peak at 2 h, then decreased but still remained above the control level at 4 h following thermal injury. The results for TBHP-initiated chemiluminescence from visceral organs following acute thermal injury were much higher than that of the control rabbits. The effects of lucigenin-initiated tissue chemiluminescence following acute thermal injury were not statistically significant. It is suggested that the decreased vascular antioxidant activity following local thermal injury is partially contributed by the superoxide pathway; while, the remote pathophysiologic events are mediated by the defective scavenging defenses. |
| Databáze: | OpenAIRE |
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