USA300-related methicillin-resistant Staphylococcus aureus clone is the predominant cause of community and hospital MRSA infections in Colombian children
| Autor: | Martha I. Alvarez-Olmos, Sandra Celina Mujica, Ana Cristina Mariño, Esther Rocio Barrero, Ricaurte Alejandro Marquez-Ortiz, Javier Antonio Escobar Pérez, Aura Lucía Leal, Betsy Esperanza Castro, Natasha Vanegas, Sebastián Gaines |
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| Jazyk: | angličtina |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical) Clonal replacement Virulence CG-MRSA Microbial Sensitivity Tests Biology CO-MRSA Colombia medicine.disease_cause Microbiology Arginine catabolic mobile element medicine Pulsed-field gel electrophoresis Humans Prospective Studies Typing Child Children Cross Infection Molecular epidemiology General Medicine USA300 Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Community-Acquired Infections Infectious Diseases Staphylococcus aureus Child Preschool Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | International Journal of Infectious Diseases. :88-93 |
| ISSN: | 1201-9712 |
| DOI: | 10.1016/j.ijid.2014.01.008 |
| Popis: | Summary Objective Community-genotype methicillin-resistant Staphylococcus aureus (CG-MRSA) isolates are known to be more virulent and clinically aggressive in children. The goal of the present study was characterize the molecular epidemiology of MRSA isolates causing infections in Colombian children. Methods An observational and prospective study was conducted between April 2009 and June 2011 at 15 hospitals in Bogota, Colombia. A detailed epidemiological profile was made of 162 children infected with MRSA. The isolates were subjected to antimicrobial susceptibility testing, molecular characterization including 21 virulence genes, SCC mec , spa and agr typing, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Results Among all isolates included in the study, 85.8% were obtained from patients whose infectious process was initiated in the community; of these, 69,8% occurred in patients without healthcare-associated risk factors. The molecular characterization of the isolates showed a high proportion (95.1%) containing a community-genotype profile with a high prevalence of SCC mec type IV, PVL-positives, and also related to CC8. Most CG-MRSA isolates (143, 92.9%) were genetically related to the pandemic clone USA300, differing by the presence of SCC mec IVc and the absence of the arginine catabolic mobile element (ACME). Conclusions An increase in the frequency of CG-MRSA infections has been reported worldwide. In this study we found that almost all MRSA infections in our pediatric population were caused by community-genotype isolates, supporting the success of the CG-MRSA clones. |
| Databáze: | OpenAIRE |
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