Functional testosterone receptors in plasma membranes of T cells
| Autor: | W. Peter M. Benten, Frank Wunderlich, Michèle Lieberherr, Christian Wrehlke, Olaf Stamm, Constantin E. Sekeris, Günter Giese, Horst Mossmann |
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| Rok vydání: | 2008 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL Receptors Antigen T-Cell CD8-Positive T-Lymphocytes Biochemistry Flow cytometry Mice Antigen Cell surface receptor Internal medicine Genetics medicine Extracellular Tumor Cells Cultured Animals Humans Testosterone Receptor Molecular Biology GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) ComputingMilieux_MISCELLANEOUS Binding Sites medicine.diagnostic_test Chemistry Cell Membrane Serum Albumin Bovine Androgen receptor Mice Inbred C57BL Endocrinology Receptors Androgen Biophysics Calcium Membrane androgen receptor CD8 Biotechnology |
| Zdroj: | FASEB Journal 13:1(Jan1999):123-133 The FASEB Journal |
| Popis: | T cells are considered to be unresponsive to testosterone due to the absence of androgen receptors (AR). Here, we demonstrate the testosterone responsiveness of murine splenic T cells in vitro as well as the presence of unconventional cell surface receptors for testosterone and classical intracellular AR. Binding sites for testosterone on the surface of both CD4(+) and CD8(+) subsets of T cells are directly revealed with the impeded ligand testosterone-BSA-FITC by confocal laser scanning microscopy (CLSM) and flow cytometry, respectively. Binding of the plasma membrane impermeable testosterone-BSA conjugate induces a rapid rise ( |
| Databáze: | OpenAIRE |
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