Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway
| Autor: | Marie F. Gorwa-Grauslund, Henrik Almqvist, Lisa Wasserstrom, Gunnar Lidén, Diogo Portugal-Nunes, Anders G. Sandström |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Biotechnology Saccharomyces cerevisiae lcsh:QR1-502 Biophysics Xylose Pentose phosphate pathway Applied Microbiology and Biotechnology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound lcsh:TP248.13-248.65 Caulobacter crescentus Glycolysis chemistry.chemical_classification d-Xylonate dehydratase biology Iron–sulfur clusters biology.organism_classification d-Xylose Yeast Weimberg pathway Citric acid cycle 030104 developmental biology Enzyme chemistry Biochemistry Original Article |
| Zdroj: | AMB Express AMB Express, Vol 8, Iss 1, Pp 1-16 (2018) |
| ISSN: | 2191-0855 |
| DOI: | 10.1186/s13568-018-0564-9 |
| Popis: | Engineering of the yeast Saccharomyces cerevisiae towards efficient d-xylose assimilation has been a major focus over the last decades since d-xylose is the second most abundant sugar in nature, and its conversion into products could significantly improve process economy in biomass-based processes. Up to now, two different metabolic routes have been introduced via genetic engineering, consisting of either the isomerization or the oxido-reduction of d-xylose to d-xylulose that is further connected to the pentose phosphate pathway and glycolysis. In the present study, cytosolic d-xylose oxidation was investigated instead, through the introduction of the Weimberg pathway from Caulobacter crescentus in S. cerevisiae. This pathway consists of five reaction steps that connect d-xylose to the TCA cycle intermediate α-ketoglutarate. The corresponding genes could be expressed in S. cerevisiae, but no growth was observed on d-xylose indicating that not all the enzymes were functionally active. The accumulation of the Weimberg intermediate d-xylonate suggested that the dehydration step(s) might be limiting, blocking further conversion into α-ketoglutarate. Although four alternative dehydratases both of bacterial and archaeon origins were evaluated, d-xylonate accumulation still occurred. A better understanding of the mechanisms associated with the activity of dehydratases, both at a bacterial and yeast level, appears essential to obtain a fully functional Weimberg pathway in S. cerevisiae. Electronic supplementary material The online version of this article (10.1186/s13568-018-0564-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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