Influence of lead ions on cation permeability in human red cell ghosts
| Autor: | T. J. B. Simons |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Quinidine
Cell Membrane Permeability Time Factors Physiology viruses Biophysics chemistry.chemical_element Buffers Calcium Ion medicine Humans Chromatography Erythrocyte Membrane Sodium virus diseases Cell Biology biochemical phenomena metabolism and nutrition Membrane transport Rubidium Red blood cell medicine.anatomical_structure Lead chemistry Permeability (electromagnetism) Potassium Efflux Intracellular medicine.drug |
| Zdroj: | The Journal of Membrane Biology. 84:61-71 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/bf01871648 |
| Popis: | Intracellular Pb2+ ions can replace Ca2+ ions in stimulating the Ca-dependent K permeability of human red blood cells. In metabolically depleted resealed ghosts, the threshold for stimulation of 86Rb efflux by internal Pb2+ is around 5 X 10(-10) M, and stimulation is half-maximal at about 2 X 10(-9) M, and maximal at 10(-8) M Pb2+. There is no effect on 22Na efflux in this concentration range. 86Rb efflux is antagonized by internal Mg2+ ions, and by the channel-blocking drugs quinidine and diS-C2(5), as observed for the Ca-dependent K permeability in red cells. In ghosts containing EDTA, which prevents any internal effects of Pb2+ ions, external Pb2+ increases both 22Na and 86Rb permeability when its concentration exceeds 6 X 10(-7) M. This effect is seemingly unrelated to the Ca-dependent K permeability. This work makes extensive use of Pb2+ ion buffers, and gives information about their preparation and properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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