The influence of fatty acids on theophylline binding to human serum albumin. Comparative fluorescence study
| Autor: | K. Sosada, A. Góra, Tomasz Wielkoszyński, Barbara Bojko, Anna Sułkowska, M. Dubas, J. Równicka-Zubik, M. Maciążek-Jurczyk, W. Żurawiński, I. Korzonek-Szlacheta, A. Szkudlarek-Haśnik, I. Zubik-Skupień |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular medicine.medical_treatment Serum albumin Analytical Chemistry chemistry.chemical_compound Theophylline medicine Humans Bovine serum albumin Instrumentation Serum Albumin Spectroscopy Binding Sites biology Fatty Acids Albumin Transporter Human serum albumin Atomic and Molecular Physics and Optics Bronchodilator Agents Spectrometry Fluorescence Biochemistry chemistry DHSA biology.protein Diuretic Protein Binding medicine.drug |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 89:270-275 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2011.12.035 |
| Popis: | Theophylline, popular diuretic, is used to treat asthma and bronchospasm. In blood it forms complexes with albumin, which is also the main transporter of fatty acids. The aim of the present study was to describe the influence of fatty acids (FA) on binding of theophylline (Th) to human serum albumin (HSA) in the high affinity binding sites. Binding parameters have been obtained on the basis of the fluorescence analysis. The data obtained for the complex of Th and natural human serum albumin (nHSA) obtained from blood of obese patients qualified for surgical removal of stomach was compared with our previous studies on the influence of FA on the complex of Th and commercially available defatted human serum albumin (dHSA). |
| Databáze: | OpenAIRE |
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