Biochemical responses of common bean to white mold potentiated by phosphites
| Autor: | A.T.H. Oliveira, I. R. F. Fagundes-Nacarath, Fabrício Ávila Rodrigues, Daniel Debona, Caroline Hawerroth |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phosphites Antioxidant Physiology medicine.medical_treatment chemistry.chemical_element Plant Science Zinc Lignin Phaseolus vulgaris 01 natural sciences Antioxidants Superoxide dismutase 03 medical and health sciences Ascomycota Phenols Defence-related enzymes Superoxides Malondialdehyde parasitic diseases Genetics medicine Food science Antioxidant system Plant Diseases Phaseolus chemistry.chemical_classification Analysis of Variance Principal Component Analysis Reactive oxygen species biology Sclerotinia sclerotiorum Hydrogen Peroxide biology.organism_classification 030104 developmental biology Solubility chemistry Catalase Thioglycolates Chitinase biology.protein 010606 plant biology & botany Peroxidase |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 0981-9428 |
| Popis: | Considering that the mechanisms for phosphite-afforded disease control remain elusive, this study investigated whether zinc (Zn) and copper (Cu) phosphites could possible potentiate common bean resistance to white mold, caused by Sclerotinia sclerotiorum, through the stimulation of biochemical defence responses. Lesion area and disease severity were decreased by phosphites spray, but Zn phosphite outcompeted Cu phosphite. Histopathological observations revealed fewer fungal hyphae and less collapse of the mesophyll cells in the Zn and Cu phosphite-sprayed plants compared to water-sprayed ones. The S. sclerotiorum-triggered accumulation of reactive oxygen species, oxalic acid (a fungal secreted toxin) and malondialdehyde (an indicator of cellular damage) were constrained as a result of Zn and Cu phosphites spray. Activities of antioxidant enzymes (superoxide dismutase, peroxidase, ascorbate peroxidase and glutathione-S-transferase at 12 h after inoculation (hai) and catalase at 60 and 84 hai) were higher for Zn and Cu phosphites-sprayed plants than for water-sprayed ones. Activities of defence-related enzymes chitinase (CHI) at 12 hai, β-1,3-glucanase (GLU) and polyphenoloxidase (PPO) were higher at 12–84 hai for Zn, and Cu phosphites sprayed plants, phenylalanine ammonia-lyase at 36–84 hai for the Zn phosphite sprayed ones, CHI at 12–36 hai, GLU at 12–60 hai, PPO at 36 hai and PAL and lipoxygenase at 12 hai for the Cu phosphite sprayed ones upon inoculation with S. sclerotiorum relative to their water-sprayed counterparts. Concentrations of total soluble phenols and lignin-thioglycolic acid derivatives were not affected by Cu phosphite spray on infected plants but were higher and lower, respectively, for Zn phosphite sprayed plants at 60 hai compared to water-sprayed ones. Taken together, the findings from the present study shed light on the biochemical defence mechanisms involved in the Zn and Cu phosphites-mediated suppression of white mold in common bean. |
| Databáze: | OpenAIRE |
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