Molecular analysis of the sex chromosomes of the platyfish Xiphophorus maculatus: towards the identification of a new type of master sexual regulator in vertebrates

Autor: Yvonne Selz, Qingchun Zhou, Sylvie Bernard-Samain, Frédéric Brunet, Manfred Schartl, Alexander Froschauer, Béatrice Segurens, Catherine Ozouf-Costaz, Jean Nicolas Volff, Cornelia Schmidt, Agnès Dettai, Stefan Chilmonczyk, Valérie Barbe, Amandine Darras, Delphine Galiana-Arnoux, Arnaud Couloux, Marta Tomaszkiewicz, Astrid Böhne, Marie Sémon, Christina Schultheis
Přispěvatelé: Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), University of Würzburg, Muséum national d'Histoire naturelle (MNHN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), German Bundesministerium fur Bildung und Forschung (BMBF), Association pour la Recherche contre le Cancer (ARC), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Superieure de Lyon (ENSL), Fondation pour la Recherche Medicale (FRM), Agence Nationale de la Recherche (ANR) et French Ministere de l'Enseignement superieur et de la Recherche, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Integrative Zoology
Integrative Zoology, 2009, 4 (3), pp.277-284. ⟨10.1111/j.1749-4877.2009.00166.x⟩
Integrative Zoology, Wiley, 2009, 4 (3), pp.277-284. ⟨10.1111/j.1749-4877.2009.00166.x⟩
ISSN: 1749-4877
DOI: 10.1111/j.1749-4877.2009.00166.x⟩
Popis: International audience; In contrast to mammals and birds, fish display an amazing diversity of genetic sex determination systems, with frequent changes during evolution possibly associated with the emergence of new sex chromosomes and sex-determining genes. To better understand the molecular and evolutionary mechanisms driving this diversity, several fish models are studied in parallel. Besides the medaka (Oryzias latipes Temminck and Schlegel, 1846) for which the master sex-determination gene has been identified, one of the most advanced models for studying sex determination is the Southern platyfish (Xiphophorus maculatus, Gunther 1966). Xiphophorus maculatus belongs to the Poeciliids, a family of live-bearing freshwater fish, including platyfish, swordtails and guppies that perfectly illustrates the diversity of genetic sex-determination mechanisms observed in teleosts. For X. maculatus, bacterial artificial chromosome contigs covering the sex-determination region of the X and Y sex chromosomes have been constructed. Initial molecular analysis demonstrated that the sex-determination region is very unstable and frequently undergoes duplications, deletions, inversions and other rearrangements. Eleven gene candidates linked to the master sex-determining gene have been identified, some of them corresponding to pseudogenes. All putative genes are present on both the X and the Y chromosomes, suggesting a poor degree of differentiation and a young evolutionary age for platyfish sex chromosomes. When compared with other fish and tetrapod genomes, syntenies were detected only with autosomes. This observation supports an independent origin of sex chromosomes, not only in different vertebrate lineages but also between different fish species.
Databáze: OpenAIRE
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