Immunogenic activity of the fish tapewormPterobothrium heteracanthum(Trypanorhyncha: Pterobothriidae) in BALB/c mice
| Autor: | S.C. São Clemente, Maurício Afonso Verícimo, Leila Maria Silva Lopes, Danuza Pinheiro Bastos Garcia de Mattos |
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| Rok vydání: | 2013 |
| Předmět: |
Mice
Inbred BALB C biology Immunogenicity Cestoda Antibodies Helminth Helminth Proteins General Medicine Sciaenidae biology.organism_classification Immunoglobulin E Trypanorhyncha Perciformes Microbiology BALB/c Fish Diseases Hookworm Infections Mice Immunization biology.protein Animals Animal Science and Zoology Parasitology Inducer |
| Zdroj: | Journal of Helminthology. 89:203-207 |
| ISSN: | 1475-2697 0022-149X |
| Popis: | The aim of this study was to verify the immunogenicity ofPterobothrium heteracanthum(Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained fromMicropogonias furnieri(Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response. |
| Databáze: | OpenAIRE |
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