PVX-tolerant potato development using a nucleic acid-hydrolyzing recombinant antibody
| Autor: | Sang-Ho Cho, K.-H. Hwang, J.-G. Yang, Eui-Joon Kil, Y. Rhee, Jungan Park, Y.-G. Lee, Sukchan Lee, Chung-Kyun Auh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Transgene Potyvirus Biology Antibodies Viral Virus law.invention 03 medical and health sciences chemistry.chemical_compound Transformation Genetic law Virology Genetic Predisposition to Disease Gene Plant Diseases Solanum tuberosum fungi food and beverages RNA General Medicine respiratory system Plants Genetically Modified Potato virus X biology.organism_classification Recombinant Proteins 030104 developmental biology Infectious Diseases chemistry Recombinant DNA Nucleic acid DNA |
| Zdroj: | Acta virologica. 61:105-115 |
| ISSN: | 1336-2305 |
| DOI: | 10.4149/av_2017_01_105 |
| Popis: | 3D8 scFv, a catalytic recombinant antibody developed in the MRL mouse, exhibits nucleic acid-hydrolyzing activity. Previous studies have demonstrated that tobacco plants harboring 3D8 scFv antibodies showed broad-spectrum resistance to infection by both DNA and RNA viruses. In this study, potatoes were transformed with the 3D8 scFv gene and screened by potato virus X (PVX) challenge. Starting with the T0 and T1 potato lines, PVX-tolerant T1 potatoes were identified in the field and characterized by ELISA and RT-PCR analysis. T2 potatoes were propagated for T3 generation and additional virus challenges in the field, and 44% of the 3D8 scFv T3 transgenic potatoes grown in GMO fields were found to be tolerant to PVX infection. Tubers from PVX-tolerant T3 lines were 60% bigger and 24% heavier, compared with tubers from PVX-susceptible transgenic lines and wild-type potatoes. Three-step virus challenge experiments and molecular characterization techniques were used for plants grown in growth chambers or fields to identify 3D8 scFv-transgenic, PVX-tolerant potatoes. These studies also revealed that the viral tolerance enabled by 3D8 scFv persisted during asexual propagation. |
| Databáze: | OpenAIRE |
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