IgG avidity index and complete blood count as biomarkers of clinical disease in naturally infected dogs with Leishmania infantum
| Autor: | Gustavo Fontes Paz, Heber Paulino Pena, Clovis Gomes de Carvalho Júnior, Rosy Iara Maciel de Azambuja Ribeiro, Marina Bellico Coelho, Eduardo Silva, Natália Ribeiro Alves, Guilherme Loureiro Werneck, Valeriana Valadares Lopes, Rafael Gonçalves Teixeira-Neto, Diego Andrade Pereira, Vinícius Silva Belo |
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| Rok vydání: | 2018 |
| Předmět: |
040301 veterinary sciences
Lymphocyte 030231 tropical medicine Population Disease Biology 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Dogs medicine Animals Onychogryphosis Dog Diseases Leishmania infantum education education.field_of_study General Veterinary medicine.diagnostic_test Complete blood count 04 agricultural and veterinary sciences General Medicine Eosinophil biology.organism_classification medicine.disease Blood Cell Count medicine.anatomical_structure Logistic Models Parasitic disease Immunology Multivariate Analysis Leishmaniasis Visceral Parasitology Biomarkers |
| Zdroj: | Veterinary parasitology. 261 |
| ISSN: | 1873-2550 |
| Popis: | Canine visceral leishmaniosis (CVL), a parasitic disease caused by Leishmania infantum, may evolve to a chronic condition and lead to death. Evaluation of infected dogs is important to establish the clinical and laboratory parameters involved in the evolution of the disease. The objectives of the present study were to discriminate a canine population (n = 52) into sub-clinical and clinically affected dogs based on signs and scores, to evaluate the hematological, biochemical, histopathological and parasitological parameters of the two dog groups, and to analyze the results by multivariate regression analysis with the aim of establishing biomarkers of CVL clinical disease. The most common signs observed in the clinically affected dogs (n = 29) were hyperkeratosis, weight loss, onychogryphosis, pale mucosa and lymphadenomegaly. In the multivariate analysis, animals presenting high IgG avidity index and low red blood, lymphocyte and eosinophil counts, and low serum urea concentration had an increased probability of being classified as clinically affected (p 0.05). All five parameters were considered to be strong biomarkers for monitoring the clinical disease, while IgG avidity percentage was strongly correlated with the number of clinical signs and could function as an indicator of the duration of infection. This is the first report on the application of IgG avidity and of multivariate regression analysis in establishing associations between the clinical signs of CVL and host biomarkers. Since avidity index (AI) percentages were strongly correlated with the number of clinical signs, it could be useful in clinical practice for auxiliary diagnosis of CVL and monitoring disease progression. A limitation of this study is the lack of information on co-infections by Anaplasma platys, Babesia canis vogeli, Ehrlichia canis and Hepatozoon canis. Therefore future studies should evaluate the influence of such co-infections on the associations studied using multivariate methods with larger samples. |
| Databáze: | OpenAIRE |
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