Effects of Vitrification on Outcomes of In VivoMature, In Vitro-Mature and Immature Human Oocytes
| Autor: | Wenyan Song, Xiangyang Zhang, Guidong Yao, Ying-Pu Sun, Xue-mei Chen, Zhao-Feng Peng, Haixia Jin, Senlin Shi, Hong-Yi Yang |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty Physiology In vivo-mature human oocytes Embryonic Development Aneuploidy Biology Group A lcsh:Physiology Cryopreservation Group B lcsh:Biochemistry Andrology Young Adult 03 medical and health sciences 0302 clinical medicine In vivo Freezing medicine Humans lcsh:QD415-436 Vitrification Zona pellucida In Situ Hybridization Fluorescence Gynecology 030219 obstetrics & reproductive medicine lcsh:QP1-981 Embryogenesis In vitro-mature human oocytes medicine.disease Immature human oocytes 030104 developmental biology medicine.anatomical_structure Oocytes Female Infertility Female |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 38, Iss 5, Pp 2053-2062 (2016) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: To observe the effects of vitrification on spindle, zona pellucida, embryonic aneuploidy and DNA injury in in vivo-maruted, in vitro-mature and immature human oocytes. Methods: Between January 2009 and February 2015, 223 immature oocytes from 450 infertile patients, and 31 in vivo-mature oocytes from 3 infertile couples were collected. Of the 223 immature oocytes, 113 were used for in vitro culture before vitrification. Some oocytes were randomly divided into in vivo-mature group (group A, n = 15), in vitro-mature group (group B, n = 88) and immature group (group C, n = 85), and then the oocytes with spindle in these three groups after freezing-thawing were selected to use for Polscope imaging, embryonic aneuploidy screening and embryo development evaluation. Other oocytes were randomly divided into group A (n = 16), group B (n = 25) and group C (n = 25) for detecting DNA injury. Results: After thawing, spindle occurrence rate, spindle Retardance value, and cleavage rate were significantly higher in groups A and B than in group C (all P < 0.05), but there were no statistical differences in fertility rate, high-quality embryo rate, blastulation rate and aneuploidy rate amongst the three groups (all P > 0.05). Zona pellucida density (ZPD) was significantly lower in group A than in groups B and C both before and after vitrification (all P < 0.05). ZPD was significantly higher after thawing than before vitrification (all P < 0.05), but zona pellucida thickness (ZPT) was not significantly changed in all the three groups (all P > 0.05). Rate of comet cells was significantly lower in group A than in groups B and C (all P < 0.01). Comet tail was significantly longer in group C than in groups B and A (all P < 0.05). Conclusion: In vivo- and in vitro-mature human oocytes are more suitable to vitrification than immature human oocytes. Spindle Retardance value has more predictive value for embryonic development potential than ZPD and ZPT. |
| Databáze: | OpenAIRE |
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