KRAS, NRAS, BRAF mutation comparison of endoscopic and surgically removed primary CRC paired samples: is endoscopy biopsy material adequate for molecular evaluation?
| Autor: | X Saegart, V. De Vriendt, Z Saridaki, G. De Hertogh, Sabine Tejpar, Dora Hatzidaki, L. De Smedt, Sofie Palmans |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Proto-Oncogene Proteins B-raf Neuroblastoma RAS viral oncogene homolog Cancer Research medicine.medical_specialty Pathology Class I Phosphatidylinositol 3-Kinases Colorectal cancer Biopsy DNA Mutational Analysis Colonoscopy colorectal cancer Adenocarcinoma Biology Real-Time Polymerase Chain Reaction medicine.disease_cause GTP Phosphohydrolases Phosphatidylinositol 3-Kinases medicine Humans Molecular Diagnostics Aged Aged 80 and over Mutation medicine.diagnostic_test Gene Expression Profiling endoscopic/primary samples Membrane Proteins Middle Aged medicine.disease Genes ras Oncology RAS/BRAF Female Histopathology KRAS Radiology Colorectal Neoplasms |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Background: An everyday clinical practice dilemma in the 20–30% of metastatic colorectal cancer (CRC) patients that have not been operated on their primary tumour, is, under which specific histopathology and molecular circumstances, an endoscopic biopsy could be considered adequate to provide a representative RAS/BRAF molecular status to guide treatment. Methods: A consecutive series of 193 paired biopsy and primary CRC tumour samples between August 2008 and 2010 available in the Department of Pathology archives, University Hospitals, KU Leuven were retrieved. For a pair to be included, in the endoscopic biopsy, 20% of invasive adenocarcinoma cells should be present and enough slides to yield an extracted DNA concentration of ⩾5 ng μl−1, and no |
| Databáze: | OpenAIRE |
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