High temperature inhibits ascorbate recycling and light stimulation of the ascorbate pool in tomato despite increased expression of biosynthesis genes
| Autor: | Rebecca Stevens, Capucine Massot, Doriane Bancel, V. Truffault, Hélène Gautier, Pierre Baldet, Félicie Lopez Lauri |
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| Přispěvatelé: | Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), EA 4279 Laboratoire Physiologie des Fruits et Légumes, Avignon Université (AU), Génétique et Amélioration des Fruits et Légumes (GAFL), Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Plantinov'Ser fundings (Pays de Loire, France), PHD grant for Capucine Massot |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Hot Temperature Light acide ascorbique lcsh:Medicine Ascorbic Acid medicine.disease_cause biosynthèse 01 natural sciences qualité nutritionnelle chemistry.chemical_compound tomate plante fruitière Gene Expression Regulation Plant Gene expression vitamine c lcsh:Science 2. Zero hunger chemistry.chemical_classification 0303 health sciences Multidisciplinary Vegetal Biology food and beverages Glutathione Biochemistry solanum lycopersicum développement du fruit Oxidation-Reduction Research Article expression des gènes fruit charnu Oxidative phosphorylation Biology 03 medical and health sciences physiologie végétale Biosynthesis medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology RNA Messenger 030304 developmental biology Vitamin C lcsh:R ascorbate APX enzyme Enzyme chemistry Fruit lcsh:Q haute température Oxidative stress Biologie végétale 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 8, Iss 12, p e84474 (2013) Plos One 12 (8), 13 p.. (2013) PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (12), 13 p. ⟨10.1371/journal.pone.0084474⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0084474⟩ |
| Popis: | International audience; Understanding how the fruit microclimate affects ascorbate (AsA) biosynthesis, oxidation and recycling is a great challenge in improving fruit nutritional quality. For this purpose, tomatoes at breaker stage were harvested and placed in controlled environment conditions at different temperatures (12, 17, 23, 27 and 31 °C) and irradiance regimes (darkness or 150 µmol m(-2) s(-1)). Fruit pericarp tissue was used to assay ascorbate, glutathione, enzymes related to oxidative stress and the AsA/glutathione cycle and follow the expression of genes coding for 5 enzymes of the AsA biosynthesis pathway (GME, VTC2, GPP, L-GalDH, GLDH). The AsA pool size in pericarp tissue was significantly higher under light at temperatures below 27 °C. In addition, light promoted glutathione accumulation at low and high temperatures. At 12 °C, increased AsA content was correlated with the enhanced expression of all genes of the biosynthesis pathway studied, combined with higher DHAR and MDHAR activities and increased enzymatic activities related to oxidative stress (CAT and APX). In contrast, at 31 °C, MDHAR and GR activities were significantly reduced under light indicating that enzymes of the AsA/glutathione cycle may limit AsA recycling and pool size in fruit pericarp, despite enhanced expression of genes coding for AsA biosynthesis enzymes. In conclusion, this study confirms the important role of fruit microclimate in the regulation of fruit pericarp AsA content, as under oxidative conditions (12 °C, light) total fruit pericarp AsA content increased up to 71%. Moreover, it reveals that light and temperature interact to regulate both AsA biosynthesis gene expression in tomato fruits and AsA oxidation and recycling. |
| Databáze: | OpenAIRE |
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