Bioorthogonal in Situ Hydrogels Based on Polyether Polyols for New Biosensor Materials with High Sensitivity
| Autor: | Rainer Haag, Uwe Schedler, Pradip Dey, Lena Kaufmann, Anna Herrmann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Azides
Materials science Polymers Nanotechnology Biosensing Techniques macromolecular substances 02 engineering and technology 010402 general chemistry complex mixtures 01 natural sciences Monolayer General Materials Science chemistry.chemical_classification Oligonucleotide Biomolecule technology industry and agriculture Hydrogels 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Covalent bond Self-healing hydrogels Click chemistry Click Chemistry Bioorthogonal chemistry 0210 nano-technology Biosensor |
| Zdroj: | ACS Applied Materials & Interfaces. 10:11382-11390 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b01860 |
| Popis: | Both noncovalent and covalent encapsulations of active biomolecules, for example, proteins and oligonucleotides, for a new biosensor matrix in an in situ bioorthogonal hydrogel formation via a strain-promoted azide-alkyne cycloaddition reaction were investigated. Unspecific interaction between the gel and the biomolecules as well as protein denaturation was prevented by the bioorthogonal gel components, which ensure a uniform aqueous environment in the hydrogel network. No leaching of the active biomolecules was observed. Additionally, a much higher and also adjustable loading of biomolecules in the hydrogel matrix was achieved compared to conventional biosensor surfaces, where the sensor molecules are immobilized on monolayers (2D surfaces) or brushlike structures (3D surfaces). Spotting experiments of the hydrogel confirm the possibility to use this new surface for microarray-based multiplex applications which require very high signal-to-noise ratios. |
| Databáze: | OpenAIRE |
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