Derivation of Intermediate Pluripotent Stem Cells Amenable to Primordial Germ Cell Specification
| Autor: | Leqian Yu, Pablo J. Ross, Noriko Tanaka, Masahiro Sakurai, Jie Li, Carlos A. Pinzon Arteaga, Hai Xi Sun, Ahmed K. Mahdi, Aoi Kohara, Canbin Zheng, Ying Gu, Jun Wu, Daiji Okamura, A. Mutto, Emily D. Ballard, Daniel A. Schmitz, Yulei Wei |
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| Rok vydání: | 2019 |
| Předmět: |
Pluripotent Stem Cells
Cell Biology Fibroblast growth factor 03 medical and health sciences Chimera (genetics) Mice 0302 clinical medicine Genetics medicine Animals Horses Induced pluripotent stem cell 030304 developmental biology 0303 health sciences Chimera Wnt signaling pathway Cell Differentiation Cell Biology Phenotype Cell biology medicine.anatomical_structure Germ Cells Molecular Medicine Stem cell 030217 neurology & neurosurgery Transforming growth factor |
| Zdroj: | Cell stem cell. 28(3) |
| ISSN: | 1875-9777 |
| Popis: | Summary Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and are capable of contributing to intra- or inter-species chimeras in vivo. XPSCs represent a pluripotency state between naive and primed pluripotency and harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency. XPSCs open new avenues for studying mammalian pluripotency and dissecting the molecular mechanisms governing PGC specification. Our method may be broadly applicable for the derivation of analogous stem cells from other mammalian species. |
| Databáze: | OpenAIRE |
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