Recent behavioral history modifies coupling between cell activity and Arc gene transcription in hippocampal CA1 neurons
| Autor: | Paul F. Worley, John F. Guzowski, Jennifer L. Sanderson, Monica K. Chawla, Bruce L. McNaughton, Peter Lipa, Levi I. Maes, Frank P. Houston, Carol A. Barnes, Teiko Miyashita |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Time Factors Transcription Genetic Recombinant Fusion Proteins Nerve Tissue Proteins Motor Activity Hippocampal formation Stimulus (physiology) Biology Primary transcript Hippocampus Memory Transcription (biology) Peripheral Nervous System Metaplasticity Neuroplasticity Image Processing Computer-Assisted Animals RNA Messenger Habituation Genes Immediate-Early Alleles In Situ Hybridization Fluorescence Neurons Genetics Microscopy Confocal Models Statistical Neuronal Plasticity Multidisciplinary Models Genetic Biological Sciences Rats Inbred F344 Rats Electrophysiology Cytoskeletal Proteins Neuroscience |
| Zdroj: | Proceedings of the National Academy of Sciences. 103:1077-1082 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0505519103 |
| Popis: | The ability of neurons to alter their transcriptional programs in response to synaptic input is of fundamental importance to the neuroplastic mechanisms underlying learning and memory. Because of technical limitations of conventional gene detection methods, the current view of activity-dependent neural transcription derives from experiments in which neurons are assumed quiescent until a signaling stimulus is given. The present study was designed to move beyond this static model by examining how earlier episodes of neural activity influence transcription of the immediate–early gene Arc . Using a sensitive FISH method that detects primary transcript at genomic alleles, the proportion of hippocampal CA1 neurons that activate transcription of Arc RNA was constant at ≈40% in response to both a single novel exploration session and daily sessions repeated over 9 days. This proportion is similar to the percentage of active neurons defined electrophysiologically. However, this close correspondence was disrupted in rats exposed briefly, but repeatedly, to the same environment within a single day. Arc transcription in CA1 neurons declined dramatically after as few as four 5-min sessions, despite stable electrophysiological activity during all sessions. Additional experiments indicate that the decrement in Arc transcription occurred at the cellular, rather than synaptic level, and was not simply linked to habituation to novelty. Thus, the neural genomic response is governed by recent, but not remote, cell firing history in the behaving animal. This state-dependence of neuronal transcriptional coupling provides a mechanism of metaplasticity and may regulate capacity for synaptic modification in neural networks. |
| Databáze: | OpenAIRE |
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