Apolipoprotein E gene polymorphism: effects on plasma lipids and risk of type 2 diabetes and coronary artery disease

Autor: Rajesh Chaudhary, Suphachai Ratanamaneechat, Atip Likidlilid, Damras Tresukosol, Thavatchai Peerapatdit, Sorachai Srisuma, Charn Sriratanasathavorn
Rok vydání: 2012
Předmět:
Apolipoprotein E
Male
medicine.medical_specialty
lcsh:Diseases of the circulatory (Cardiovascular) system
Endocrinology
Diabetes and Metabolism
Type 2 diabetes
Coronary Artery Disease
Polymerase Chain Reaction
Risk Assessment
Coronary artery disease
Apolipoproteins E
Gene Frequency
Risk Factors
Internal medicine
Diabetes mellitus
Genotype
Type 2 diabetes mellitus
medicine
Odds Ratio
Humans
Genetic Predisposition to Disease
Allele
Polymorphism
Original Investigation
Chi-Square Distribution
Polymorphism
Genetic
business.industry
nutritional and metabolic diseases
Odds ratio
Middle Aged
medicine.disease
Thailand
Lipids
Endocrinology
Logistic Models
Phenotype
Diabetes Mellitus
Type 2
lcsh:RC666-701
Case-Control Studies
Hyperglycemia
Multivariate Analysis
Female
Gene polymorphism
Cardiology and Cardiovascular Medicine
business
Restriction fragment length polymorphism
Biomarkers
Zdroj: Cardiovascular Diabetology
Cardiovascular Diabetology, Vol 11, Iss 1, p 36 (2012)
ISSN: 1475-2840
Popis: Background The most common apolipoprotein E (apoE) gene polymorphism has been found to influence plasma lipid concentration and its correlation with coronary artery disease (CAD) has been extensively investigated in the last decade. It is, however, unclear whether apoE gene polymorphism is also associated with increased risk of type 2 diabetes mellitus (T2DM). The knowledge of this study may provide the primary prevention for T2DM and CAD development before its initiation and progression. Therefore, this study was carried out to determine the association between apoE gene polymorphism and T2DM with and without CAD and its role in lipid metabolism. Methods The case-control study was carried out on a total of 451 samples including 149 normal control subjects, 155 subjects with T2DM, and 147 subjects with T2DM complicated with CAD. The apoE gene polymorphism was tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Univariable and multivariable logistic regression analyses were used to identify the possible risks of T2DM and CAD. Results A significantly increased frequency of E3/E4 genotype was observed only in T2DM with CAD group (p = 0.0004), whereas the ε4 allele was significantly higher in both T2DM (p = 0.047) and T2DM with CAD (p = 0.009) as compared with controls. E3/E4 genotype was also the independent risk in developing CAD after adjusting with established risk factors with adjusted odds ratio (OR) 2.52 (95%CI 1.28-4.97, p = 0.008). The independent predictor of individuals carrying ε4 allele still remained significantly associated with both CAD (adjusted OR 2.32, 95%CI 1.17-4.61, p = 0.016) and T2DM (adjusted OR 2.04, 95%CI 1.07-3.86, p = 0.029). After simultaneously examining the joint association of E3/E4 genotype combined with either obesity or smoking the risk increased to approximately 5-fold in T2DM (adjusted OR 4.93, 95%CI 1.74-13.98, p = 0.003) and 10-fold in CAD (adjusted OR 10.48, 95%CI 3.56-30.79, p < 0.0001). The association between apoE genotypes on plasma lipid levels was compared between E3/E3 as a reference and E4-bearing genotypes. E4-bearing genotypes showed lower HDL-C and higher VLDL-C and TG, whereas other values of plasma lipid concentrations showed no significant difference. Conclusions These results indicate that ε4 allele has influence on lipid profiles and is associated with the development of both T2DM with and without CAD, and furthermore, it increased the risk among the subjects with obesity and/or smoking, the conditions associated with high oxidative stress.
Databáze: OpenAIRE
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