Effect of steam explosion pretreatment on the structure and bioactivity of Ampelopsis grossedentata polysaccharides
| Autor: | Yun-Qin Jia, Chun-Yu Liu, Wei Jiang, Xue-Qing Geng, Bei-Yu Xie, Yang-Yang Sun, Zhen-Yuan Zhu, Li-Chao Pan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ampelopsis
ved/biology.organism_classification_rank.species Mixed type 02 engineering and technology Pretreatment method Polysaccharide Methylation Biochemistry 03 medical and health sciences Polysaccharides Structural Biology Spectroscopy Fourier Transform Infrared Humans Glycoside Hydrolase Inhibitors Surface plasmon resonance Molecular Biology 030304 developmental biology Steam explosion chemistry.chemical_classification 0303 health sciences Chromatography Chemistry ved/biology alpha-Glucosidases Biological activity General Medicine Surface Plasmon Resonance 021001 nanoscience & nanotechnology Molecular Weight Steam Carbohydrate Sequence 0210 nano-technology Ampelopsis grossedentata |
| Zdroj: | International Journal of Biological Macromolecules. 185:194-205 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.06.002 |
| Popis: | Steam explosion (SE) was a friendly environmentally pretreatment method. In this study, the effect of steam explosion (SE) pretreatment on structure and α-glucosidase inhibitory activity of Ampelopsis grossedentata polysaccharides was evaluated. Two novel polysaccharides (AGP and AGP-SE) were extracted, isolated, purified and analyzed by NMR, FT-IR and methylation. The results indicated that AGP mainly consisted of Rha, Xyl, Glc, and Ara with a molecular weight of 2.74 × 103 kDa and AGP-SE mainly consisted of Man, Ara, and Gal with a molecular weight of 2.14 × 103 kDa. Furthermore, the backbone of AGP and AGP-SE were mainly composed of 5)-Araf-(1→, -Glcp-(1→, 6)-Glcp-(1→, 6)-Galp-(1→, 3,6)-Manp-(1→, and 2,3,6)-Glcp-(1→. Finally, we demonstrated that all polysaccharides exhibited obviously α-glucosidase inhibition activity and mixed type inhibition. AGP-SE had better α-glucosidase inhibition activity and the binding affinity KD on α-glucosidase by using Surface Plasmon Resonance (SPR) than AGP. Overall, SE pretreatment is an effective method for extracting polysaccharide and provides a new idea into the improvement of biological activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect