Spectrum Effect Relationship and Component Knock-Out in Angelica Dahurica Radix by High Performance Liquid Chromatography-Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer
| Autor: | Lin-Na Peng, Jinmei Wang, Wenyi Kang, Changqin Li, Mengjun Shi, Yan Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Tyrosinase Pharmaceutical Science tyrosinase Orbitrap Mass spectrometry Bergapten High-performance liquid chromatography Plant Roots Mass Spectrometry Article component knock-out Analytical Chemistry law.invention lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound spectrum-effect relationship lcsh:Organic chemistry law Furocoumarins Drug Discovery Radix Angelica dahuricae Physical and Theoretical Chemistry Chromatography High Pressure Liquid Angelica Chromatography Plants Medicinal biology Imperatorin Plant Extracts Angelica dahurica Organic Chemistry biology.organism_classification 030104 developmental biology chemistry Chemistry (miscellaneous) Molecular Medicine Heterocyclic Compounds 3-Ring Drugs Chinese Herbal |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 7; Pages: 1231 Molecules, Vol 22, Iss 7, p 1231 (2017) |
| ISSN: | 1420-3049 |
| Popis: | Different extracts of Angelica dahuricae were available for whitening or treating vitiligo clinically. They showed inhibitory or activating effects on tyrosinase, a rate-limiting enzyme of melanogenesis. This study aimed to identify active compounds on tyrosinase in water extract of Angelica dahurica Radix. We applied spectrum-effect relationship and component knock-out methods to make it clear. HPLC was used to obtain the specific chromatograms. The effects on tyrosinase activity were examined by measuring the oxidation rate of levodopa in vitro. Partial least squares method was used to examine the spectrum-effect relationships. The knocked-out samples were prepared by HPLC method, and the identification of knocked-out compounds was conducted by the high performance liquid chromatography-four stage rod-electrostatic field orbit trap high resolution mass spectrometry. Results showed that S6, S14, S18, S21, S35, S36, S37, S40, and S41 were positively correlated to inhibitory activity of Angelica dahuricae on tyrosinase whereas S9, S11, S8, S12, S22, and S30 were negatively correlated. When the concentration of each sample was 1 g·mL-1, equal to the amount of raw medicinal herbs, oxypeucedanin hydrate, imperatorin, cnidilin, and isoimperatorin had inhibitory effects on tyrosinase activity whereas byakangelicin and bergapten had activating effects. |
| Databáze: | OpenAIRE |
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