Subclinical response to cadmium in liver cells
Autor: | Ludwig Müller, Neill H. Stacey |
---|---|
Rok vydání: | 1989 |
Předmět: |
Male
Endocrinology Diabetes and Metabolism Clinical Biochemistry Heme Mitochondrion Biochemistry Dithiothreitol Electron Transport Complex IV Inorganic Chemistry chemistry.chemical_compound In vivo Animals Metallothionein Cytochrome c oxidase Sulfhydryl Compounds biology Cytochrome c Biochemistry (medical) Rats Inbred Strains General Medicine Glutathione Molecular biology In vitro Mitochondria Rats Liver chemistry Phenobarbital biology.protein Cadmium |
Zdroj: | Biological Trace Element Research. 21:317-323 |
ISSN: | 1559-0720 0163-4984 |
Popis: | Effects of cadmium (Cd) in vivo and in vitro were studied in the absence of enhanced metallothionein (MT) production and overt Cd toxicity. Such a condition was established by extended oral exposure of male rats to 0.2 mumols Cd/kg and by incubation of isolated hepatocytes with up to 25 microM for 30 min. Subsequently, mitochondrial and extramitochondrial responses to Cd were recorded. Cadmium diminished the activity of cytochrome c oxidase (CYT C OX) by 50% in vivo and by 35% in vitro. In hepatocytes, this was accompanied by increased Cd and decreased protoheme (PrH) in mitochondria. Extramitochondrial PrH and cytochrome P 450 were not significantly altered. In hepatocytes from phenobarbitone pretreated rats, 25 microM Cd decreased CYT C OX but not mitochondrial PrH. Moreover, stimultaneous incubation of hepatocytes with 25 microM Cd and either 2.5 mM dithiothreitol or 5 mM reduced glutathione diminished cellular and mitochondrial Cd and prevented the decrease in CYT C OX but not that in PrH. In contrast, coincubation with either 250 microM L-buthionine-sulfoximine or diethylmaleate, which did not alter Cd uptake, prevented the decrease in PrH but not that in CYT C OX owing to Cd. These results show that Cd exerts mitochondrial alterations in vivo and in vitro in the absence of enhanced MT production. Moreover, Cd effects on CYT C OX and PrH do not seem to be firmly linked. |
Databáze: | OpenAIRE |
Externí odkaz: |