A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
| Autor: | Jonas Blomberg, Ronnie Eriksson, Sándor Belák, István Kiss, Ann Nordengrahn, Malik Merza, Lihong Liu, Hongyan Xia |
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| Rok vydání: | 2010 |
| Předmět: |
Antibodies
Viral Sensitivity and Specificity Article Virus Microsphere Flaviviridae Virology Luminex medicine Animals BVDV Viral diarrhoea Immunoassay Diarrhea Viruses Bovine Viral biology medicine.diagnostic_test Pestivirus Reproducibility of Results Microsphere immunoassay biology.organism_classification Molecular biology Microspheres Highly sensitive biology.protein Bovine Virus Diarrhea-Mucosal Disease Cattle Antibody |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2010.04.009 |
| Popis: | This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. |
| Databáze: | OpenAIRE |
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