Low-depth sequencing for copy number abnormalities in multiple myeloma supersedes fluorescent in situ hybridization in scope and resolution

Autor:	Mohamed Abou El Hassan, Wenda L. Greer, Nicholas A. Forward, Clinton J. V. Campbell, Samuel D. Cutler, Philipp Knopf, Manal O. Elnenaei, Julie Wagner, Daniel Gaston, Darrell White, Keaton Sinclair, Stephen Couban, Marissa Goudie
Rok vydání:	2019
Předmět:	0301 basic medicine Resolution (mass spectrometry) DNA Copy Number Variations Genomics In situ hybridization 030105 genetics & heredity Biology 03 medical and health sciences Genetics medicine Humans Genetics (clinical) Multiple myeloma In Situ Hybridization Fluorescence Whole genome sequencing Comparative Genomic Hybridization Whole Genome Sequencing Chromosome Mapping Computational Biology medicine.disease Molecular biology Fluorescence 030104 developmental biology Hematological malignancy Multiple Myeloma Size adjustment
Zdroj:	Clinical genetics. 96(2)
ISSN:	1399-0004
Popis:	Multiple myeloma (MM) is an incurable hematological malignancy that relies on cytogenetic determination of copy number abnormalities (CNAs) for prognosis and management. Low-depth whole genome sequencing (LD-WGS) is a cost-effective alternative to targeted genomics for CNA detection, but its value has yet to be explored in MM. DNA from CD138+ cells from MM patients were sequenced using an Illumina NextSeq at
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fd218a80f26316258bcbeedb1410975 https://pubmed.ncbi.nlm.nih.gov/31066036 Zobrazit plný text záznamu Plný text