Different Involvement of Band 3 in Red Cell Deformability and Osmotic Fragility—A Comparative GP.Mur Erythrocyte Study
| Autor: | Kate Hsu, Han-Chih Cheng, Jong-Shyan Wang, Mei-Shin Kuo, Hui-Ru Lin, Cheng-Hsi Chuang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Erythrocytes Osmotic shock QH301-705.5 membrane cholesterol Models Biological band 3 AQP1 (aquaporin-1) Article erythrocyte (red blood cells) GP.Mur (Miltenberger subtype III) deformability osmotic fragility microcytosis mean corpuscular volume (MCV) Anion Exchange Protein 1 Erythrocyte Erythrocyte Deformability medicine Humans Glycophorin Biology (General) Band 3 Aquaporin 1 Red Cell biology Chemistry Microcytosis Erythrocyte Membrane Erythrocyte fragility General Medicine medicine.disease Red blood cell Cholesterol medicine.anatomical_structure Multivariate Analysis Erythrocyte Count Biophysics biology.protein Regression Analysis Tonicity Protein Binding circulatory and respiratory physiology |
| Zdroj: | Cells, Vol 10, Iss 3369, p 3369 (2021) Cells; Volume 10; Issue 12; Pages: 3369 Cells |
| ISSN: | 2073-4409 |
| Popis: | GP.Mur is a clinically important red blood cell (RBC) phenotype in Southeast Asia. The molecular entity of GP.Mur is glycophorin B-A-B hybrid protein that promotes band 3 expression and band 3–AQP1 interaction, and alters the organization of band 3 complexes with Rh/RhAG complexes. GP.Mur+ RBCs are more resistant to osmotic stress. To explore whether GP.Mur+ RBCs could be structurally more resilient, we compared deformability and osmotic fragility of fresh RBCs from 145 adults without major illness (47% GP.Mur). We also evaluated potential impacts of cellular and lipid factors on RBC deformability and osmotic resistivity. Contrary to our anticipation, these two physical properties were independent from each other based on multivariate regression analyses. GP.Mur+ RBCs were less deformable than non-GP.Mur RBCs. We also unexpectedly found 25% microcytosis in GP.Mur+ female subjects (10/40). Both microcytosis and membrane cholesterol reduced deformability, but the latter was only observed in non-GP.Mur and not GP.Mur+ normocytes. The osmotic fragility of erythrocytes was not affected by microcytosis; instead, larger mean corpuscular volume (MCV) increased the chances of hypotonic burst. From comparison with GP.Mur+ RBCs, higher band 3 expression strengthened the structure of RBC membrane and submembranous cytoskeletal networks and thereby reduced cell deformability; stronger band 3–AQP1 interaction additionally supported osmotic resistance. Thus, red cell deformability and osmotic resistivity involve distinct structural–functional roles of band 3. |
| Databáze: | OpenAIRE |
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