Pro-oxidant activity and genotoxicity of the Astronium fraxinifolium using wing SMART and allium cepa test
| Autor: | Paulo Cesar Ferreira, Rafael Rozolen Teixe Zafred, Mario Antonio Spano, Regildo Marcio Goncalves, Luciana Pereira Si, Celia Cristina Malag Figueiredo, Gustavo Rafagnin Martins |
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| Přispěvatelé: | Universidade Estadual de Campinas (UNICAMP), Universidade Federal de Uberlândia (UFU), Universidade Estadual Paulista (UNESP), Fundação Educacional do Município de Assis (FEMA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Traditional medicine biology.organism_classification Pro-oxidant medicine.disease_cause 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Complementary and alternative medicine Medicinal plants Micronucleus Astronium fraxinifolium 030220 oncology & carcinogenesis Botany Oxidation medicine Allium Mutagenic tests Tannins Genotoxicity |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-29T07:35:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01 Astronium fraxinifolium is an arboreal species found throughout the Brazilian Cerrado region and used in folk medicine as antimicrobial, anti-hemorrhagic and healing. Pro-oxidant activity of extracts of fraxinifolium rough Relative Electrophoresis Mobility (REM) of Bovine Serum Albumin (BSA) protein in presence of the extract and Cu2+ and also the genotoxic potential through Somatic Mutation and Recombination Test (SMART) and Allium cepa tests has been investigated during this study. In the REM, the extracts acting exclusively on BSA resulted in a band formation with higher molecular weight than BSA, probably due to oxidative action of the extracts. In presence of Cu2+and extracts occurred protein fragmentation due to Cu2+ oxidative action potentiated by the extracts. In SMART test the frequency of mutant spots increases with the increased concentration of extract A. fraxinifolium (50 mg mL-1= 1.40 and 100 mg mL-1 = 2.66). The HB cross shows a decrease in the total of mutant spots frequency for the different treatments (50 mg mL-1 =6.60 and 100 mg mL-1 = 3.25). Evaluation of A cutest demonstrated the following results (concentration extract = chromosomal abnormalities) 1 mg mL-1 = 85,10 mg L-1 = 61 50 mg mL-1 = 53,100 mg mL-1 =33 and MMS 10 mg L-1 = 50. Genotoxic and cytotoxic actions can be explained by the actions of tannins present in its composition. Butthere may be other substances that also act for such results. The genotoxicity in medicinal plants contributes to therapeutic safety warning that although the use of medicinal plants is an inexpensive and non-aggressive method it can cause harmful effects if used incorrectly. Centro Pluridisciplinar de Pesquisas Químicas Biologica e Agrárias Divisão de Farmacologia e Toxicologia Universidade Estadual de Campinas, Av. Alexandre Cazelatto 999 Universidade Federal de Uberlândia Instituto de Genetica e Bioquímica Universidade Federal de Uberlândia Laboratorio de Fitoterápicos Departamento de Ciências Biologicas Faculdade de Ciências e Letras de Assis Universidade Estadual Paulista, Avenida Dom Antônio 2100 Fundação Educacional do Município de Assis (FEMA) Laboratorio de Fitoterápicos Departamento de Ciências Biologicas Faculdade de Ciências e Letras de Assis Universidade Estadual Paulista, Avenida Dom Antônio 2100 |
| Databáze: | OpenAIRE |
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