Flow cytometric analysis of cerebrospinal fluid samples and its usefulness in routine clinical practice
| Autor: | Carmen Jiménez-Garófano, Alberto Orfao, Jaime Hernández, Esther Aceituno, Ana M. Jiménez, Antonio García Jiménez, Alejandro Román, Dolores Subirá, Susana Castañón |
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| Rok vydání: | 2002 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cell type Pathology medicine.medical_specialty Population CD8-Positive T-Lymphocytes Sensitivity and Specificity Flow cytometry Immunophenotyping Cerebrospinal fluid T-Lymphocyte Subsets Medicine Humans Routine clinical practice education Cerebrospinal Fluid education.field_of_study B-Lymphocytes Leukemia medicine.diagnostic_test business.industry Reproducibility of Results General Medicine Flow Cytometry T cell subset Immunology business CD8 |
| Zdroj: | American journal of clinical pathology. 117(6) |
| ISSN: | 0002-9173 |
| Popis: | Low volume and few cells have hampered the use of flow cytometry for studying cerebrospinal fluid (CSF) in routine clinical practice, although information about the cellular phenotypes present in this type of sample is of great value in many diseases. We developed a novel flow cytometric strategy capable of identifying total CSF T lymphocytes and the CD4+ subset, even in CSF samples with as few as 1 leukocyte per 3 µL of sample. We also showed that identification of CD8+ T cells could be achieved in most samples, while B lymphocytes are detectable only in samples with more than 5 cells per microliter. These findings demonstrate the reliability of this method to improve the diagnostic accuracy of classic cytologic studies in many neurologic disorders. The analysis of cerebrospinal fluid (CSF) cells represents the first diagnostic approach in many inflammatory neurologic diseases. At present, the study of CSF cells is restricted almost to the analysis of their morphologic characteristics, which has several limitations for the specific identification of different lymphoid cell types. Accordingly, simultaneous use of immunophenotypic techniques could be of great help in this regard. Nevertheless, only a few markers can be assessed routinely by conventional microscopy-based immunocytochemical or immunofluorescent techniques owing to the relatively low number of cells present in this type of sample. Although flow cytometry has proved to be of great help for the evaluation of fluids containing a high number of cells, the low number of cells available has seriously limited its use for the study of the CSF. To overcome these problems, different groups have tried to collect higher CSF volumes. 1,2 However, this possibility cannot be considered for routine clinical practice. Our aim was to explore the clinical usefulness of flow cytometry immunophenotyping for the analysis of CSF samples obtained in clinical practice according to wellestablished routine criteria. First, we designed a flow cytometric approach to establish the minimum number of events required to accurately identify any given population. Then, we tested the usefulness of this approach for the reliable identification and enumeration of the major cell subsets present in CSF samples from patients with different neurologic conditions. |
| Databáze: | OpenAIRE |
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