Estrogen differentially regulates expression ofPer1 andPer2 genes between central and peripheral clocks and between reproductive and nonreproductive tissues in female rats
| Autor: | Takahiro J. Nakamura, Shizufumi Ebihara, Shin Inoue, Shigenori Watanabe, Kazuyuki Shinohara, Takahiro Moriya, Takao Shimazoe |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Period (gene) Cell Cycle Proteins Estrous Cycle Biology Kidney Cellular and Molecular Neuroscience Biological Clocks Internal medicine medicine Animals RNA Messenger Circadian rhythm Rats Wistar Cerebral Cortex Suprachiasmatic nucleus Uterus Nuclear Proteins Kidney metabolism Estrogens Period Circadian Proteins Circadian Rhythm Rats PER2 CLOCK Endocrinology Gene Expression Regulation Liver Estrogen Female Suprachiasmatic Nucleus hormones hormone substitutes and hormone antagonists Transcription Factors PER1 |
| Zdroj: | Journal of Neuroscience Research. 82:622-630 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.20677 |
| Popis: | Although it has long been established that estrogen alters circadian rhythms in behavior, physiology, and reproductive functions in mammals, the molecular mechanism for these effects remains unknown. To explore the possibility that estrogen affects circadian rhythms by changing the expression of clock-related genes, we investigated the effects of chronic treatment with 17beta-estradiol (E2) on the expression of Per1 and Per2 genes in the brain (suprachiasmatic nucleus and cerebral cortex) and periphery (liver, kidney, and uterus) of ovariectomized rats by means of in situ hybridization and northern blotting. In the brain, E2 treatment advanced the peak of Per2 mRNA expression in the SCN; however, it failed to affect the rhythm of Per2 mRNA expression in the CX and Per1 mRNA expression in both the SCN and the CX. In nonreproductive peripheral tissues (liver and kidney), E2 delayed the phase and increased the amplitude of Per1 mRNA expression. In the reproductive tissues (uterus), biphasic rhythms in Per1 and Per2 mRNA were observed after E2 treatment. These findings suggest that the effects of estrogen are different between central and peripheral clock in the brain, and between reproductive and nonreproductive tissues in the periphery. |
| Databáze: | OpenAIRE |
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