Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD
| Autor: | Yi-Jun Liu, Daxiao Cheng, Xiao-Dong Wang, Duo Duan, Shumin Duan, Huifang Lou, Liya Zhu, Junhua Yang, Margaret S. Ho, Sicong Chen, Xingyue Wang, Xia Li, Ting Zhang, Jianhong Luo |
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| Rok vydání: | 2020 |
| Předmět: |
Endosome
Cathepsin D Endosomes macromolecular substances 03 medical and health sciences 0302 clinical medicine Organelle Cytoskeleton Research Articles Actin 030304 developmental biology 0303 health sciences Multidisciplinary Chemistry SciAdv r-articles Cell Biology Cofilin Actins Cell biology Actin Cytoskeleton Cytosol Cellular Neuroscience 030220 oncology & carcinogenesis Microglia Lamellipodium Peptide Hydrolases Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.aba5783 |
| Popis: | Protease cathD escapes from late endosomes to cytosol and accelerates actin dynamics. Organelle transport requires dynamic cytoskeleton remodeling, but whether cytoskeletal dynamics are, in turn, regulated by organelles remains elusive. Here, we demonstrate that late endosomes, a type of prelysosomal organelles, facilitate actin-cytoskeleton remodeling via cytosolic translocation of immature protease cathepsin D (cathD) during microglia migration. After cytosolic translocation, late endosome–derived cathD juxtaposes actin filaments at the leading edge of lamellipodia. Suppressing cathD expression or blocking its cytosolic translocation impairs the maintenance but not the initiation of lamellipodial extension. Moreover, immature cathD balances the activity of the actin-severing protein cofilin to maintain globular-actin (G-actin) monomer pool for local actin recycling. Our study identifies cathD as a key lysosomal molecule that unconventionally contributes to actin cytoskeleton remodeling via cytosolic translocation during adenosine triphosphate–evoked microglia migration. |
| Databáze: | OpenAIRE |
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