Sensitive determination of lysozyme by using a luminescent and colorimetric probe based on the aggregation of gold nanoparticles induced by an anionic ruthenate(II) complex
| Autor: | Chi-Chiu Ko, Mei-Jin Li, Xiangjun Mu, Kin-Cheung Chan, Jing Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Chemistry
Calibration curve Inorganic chemistry Nanochemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Colloidal gold Lysozyme 0210 nano-technology Luminescence Egg white Electrostatic interaction |
| Zdroj: | Mikrochimica acta. 185(9) |
| ISSN: | 1436-5073 |
| Popis: | The negatively charged ruthenate(II) complex [Ru(bpy)(PPh3)(CN)3]− and gold nanoparticles (AuNPs) were used for detecting lysozyme (LYS). The luminescence of the ruthenate(II) complex is quenched by AuNPs, and this induces the aggregation of AuNPs and a color change from red to blue. After addition of lysozyme, the positively charged lysozyme and the negatively charged ruthenate(II) complex bind each other by electrostatic interaction firstly. This prevents AuNPs from aggregation and quenches the emission of the ruthenate(II) complex. Its luminescence and the degree of aggregation of the AuNPs can be used to quantify LYS. The fluorometric calibration plot is linear in the 0.01 to 0.20 μM LYS concentration range, and the calibration plot is linear between 0.02 and 0.20 μM of LYS. The color of the solution can be easily distinguished by bare eyes at 0.08 μM or higher concentration of LYS. The applicability of the method was verified by the correct analysis of LYS in chicken egg white. |
| Databáze: | OpenAIRE |
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