Evaluation of LAMP-based assays for carbapenemase genes
| Autor: | Lakshmi Vemu, Deepak Mendiratta, Jyoti Gupta, Chiranjay Mukhopadhyay, Reba Kanungo, Mary Dias, Anuradha De, Akshaya Jagan, Rachana Solanki, Pradeep Nawal, Sudipta Patra, Jayanthi Shastri, Vani Reddy, Karuna Patel, Rasika Venkataraman, V. Sangeetha, Gargi Deshmukh, Surobhi Lahiri, Radha Rangarajan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genetics 030106 microbiology Loop-mediated isothermal amplification General Medicine Biology Microbiology beta-Lactamases 03 medical and health sciences 030104 developmental biology Bacterial Proteins Gram-Negative Bacteria Humans Gram-Negative Bacterial Infections Nucleic Acid Amplification Techniques Gene Carbapenem resistance |
| Zdroj: | Journal of Medical Microbiology. 68:1431-1437 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Purpose: Rapid and accurate detection of carbapenem resistance is a critical requirement for the selection of appropriate therapy and initiation of infection control measures. Although several tests are available, their use is limited by one or more factors. Phenotypic tests are lengthy, have variable sensitivity and specificity and do not generally identify the carbapenemase. Molecular assays overcome many of these issues but cost can be a barrier to adoption, particularly in low-resource settings. To address the need for affordable, molecular tools, we have assessed the performance characteristics of loop-mediated isothermal amplification (LAMP)-based assays for the major carbapenemase genes, blaNDM, blaKPC, blaOXA-48, blaOXA-23 blaVIM and blaIMP. Methodology: The assays were validated using 1849 Gram-negative Indian clinical isolates obtained from seven hospitals and diagnostic centres. Results: The assays had diagnostic sensitivities of 98.14 %, 98.92 %, 100 %, 98.48 %, and diagnostic specificities of 98.94 %, 99.61 %, 97.42 %, 99.38 % for blaNDM, blaOXA-48, blaOXA-23 and blaVIM, respectively. Due to a low number of isolates positive for blaKPC and blaIMP, the performance characteristics of assays for these two genes could not be suitably evaluated. Conclusion: The performance characteristics suggest suitability for diagnostic and surveillance purposes. Intro duction The emergence of carbapenem-resistant Gram-negative infections is a major public health threat globally. The problem is particularly grave. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|