A Smart Multifunctional Nanoparticle for Enhanced Near-Infrared Image-Guided Photothermal Therapy Against Gastric Cancer
| Autor: | Hongbo Wei, Shengxue Hu, Xiaoming Zheng, Rongpu Liang, Dongbing Ding, Bo Wei, Jun Shao, Xudong Zhu |
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| Rok vydání: | 2021 |
| Předmět: |
Indocyanine Green
Photothermal Therapy Polymers Multifunctional nanoparticles Biophysics Pharmaceutical Science Bioengineering Multifunctional Nanoparticles 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound International Journal of Nanomedicine Stomach Neoplasms In vivo Cell Line Tumor Drug Discovery medicine Animals Humans Tumor growth Original Research Chitosan Mice Inbred BALB C Chemistry gastric cancer nanoparticle Organic Chemistry Cancer General Medicine Phototherapy Photothermal therapy 021001 nanoscience & nanotechnology medicine.disease Boronic Acids Xenograft Model Antitumor Assays In vitro 0104 chemical sciences near-infrared imaging Cancer cell Cancer research Female Reactive Oxygen Species Corrigendum 0210 nano-technology Oligopeptides Indocyanine green |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Jun Shao,1,* Rongpu Liang,1,* Dongbing Ding,1,* Xiaoming Zheng,1 Xudong Zhu,1 Shengxue Hu,2 Hongbo Wei,1 Bo Wei1 1Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, People’s Republic of China; 2College of Biological Science and Engineering, Fuzhou University, Fujian, 350108, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongbo Wei; Bo WeiDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, People’s Republic of ChinaTel +86-20-85252228Email weihb@mail.sysu.edu.cn; weibo3@mail.sysu.edu.cnBackground: Surgery is considered to be a potentially curative approach for gastric cancer. However, most cases are diagnosed at a very advanced stage for the lack of typical symptoms in the initial stage, which makes it difficult to completely surgical resect of tumors. Early diagnosis and precise personalized intervention are urgent issues to be solved for improving the prognosis of gastric cancer. Herein, we developed an RGD-modified ROS-responsive multifunctional nanosystem for near-infrared (NIR) imaging and photothermal therapy (PTT) against gastric cancer.Methods: Firstly, the amphiphilic polymer was synthesized by bromination reaction and nucleophilic substitution reaction of carboxymethyl chitosan (CMCh) and 4-hydroxymethyl-pinacol phenylborate (BAPE). Then, it was used to encapsulate indocyanine green (ICG) and modified with RGD to form a smart multifunctional nanoparticle targeted to gastric cancer (CMCh-BAPE-RGD@ICG). The characteristics were determined, and the targeting capacity and biosafety were evaluated both in vitro and in vivo. Furthermore, CMCh-BAPE-RGD@ICG mediated photothermal therapy (PTT) effect was studied using gastric cancer cells (SGC7901) and SGC7901 tumor model.Results: The nanoparticle exhibited suitable size (≈ 120 nm), improved aqueous stability, ROS-responsive drug release, excellent photothermal conversion efficiency, enhanced cellular uptake, and targeting capacity to tumors. Remarkably, in vivo studies suggested that CMCh-BAPE-RGD@ICG could accurately illustrate the location and margin of the SGC7901 tumor through NIR imaging in comparison with non-targeted nanoparticles. Moreover, the antitumor activity of CMCh-BAPE-RGD@ICG-mediated PTT could effectively suppress tumor growth by inducing necrosis and apoptosis in cancer cells. Additionally, CMCh-BAPE-RGD@ICG demonstrated excellent biosafety both in vitro and in vivo.Conclusion: Overall, our study provides a biocompatible theranostic nanoparticle with enhanced tumor-targeting ability and accumulation to realize NIR image-guided PTT in gastric cancer.Keywords: gastric cancer, nanoparticle, indocyanine green, near-infrared imaging, photothermal therapy |
| Databáze: | OpenAIRE |
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