MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata
| Autor: | Maria Immacolata Ferrante, Monia Teresa Russo, Remo Sanges, Marina Montresor, Filomena Romano, Laura Entrambasaguas, Carmen Minucci, Elio Biffali, Laura Vitale, Wim Vyverman, Pasquale De Luca, Konstantinos Anestis, Neri Fattorini |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
EXPRESSION
0106 biological sciences 0301 basic medicine Mating type Diatoms Phylogeny Transcriptome Science General Physics and Astronomy INHERITANCE 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Settore BIO/13 - Biologia Applicata TOOL lcsh:Science Gene reproductive and urinary physiology Regulation of gene expression Genetics Multidisciplinary biology Human evolutionary genetics 010604 marine biology & hydrobiology fungi Biology and Life Sciences General Chemistry Sex reversal biology.organism_classification EVOLUTION TIME GENOME LIFE INSIGHTS 030104 developmental biology Diatom behavior and behavior mechanisms lcsh:Q Pseudo-nitzschia |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018) Nature Communications NATURE COMMUNICATIONS |
| ISSN: | 2041-1723 |
| Popis: | A broad diversity of sex-determining systems has evolved in eukaryotes. However, information on the mechanisms of sex determination for unicellular microalgae is limited, including for diatoms, key-players of ocean food webs. Here we report the identification of a mating type (MT) determining gene for the diatom Pseudo-nitzschia multistriata. By comparing the expression profile of the two MTs, we find five MT-biased genes, of which one, MRP3, is expressed exclusively in MT+ strains in a monoallelic manner. A short tandem repeat of specific length in the region upstream of MRP3 is consistently present in MT+ and absent in MT− strains. MRP3 overexpression in an MT− strain induces sex reversal: the transgenic MT− can mate with another MT− strain and displays altered regulation of the other MT-biased genes, indicating that they lie downstream. Our data show that a relatively simple genetic program is involved in defining the MT in P. multistriata. Little is known about how sex is determined in diatoms. Here, Russo et al. show that in the diatom Pseudo-nitzschia multistriata, the gene MRP3 regulates mating type and the expression of four other mating-type biased genes. |
| Databáze: | OpenAIRE |
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