Biochemical differences in the mechanism of macrophage lysosomal exocytosis initiated by zymosan particles and weak bases
| Autor: | D R Stanworth, J L Watkins, D W H Riches |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
Male
Stimulation Deoxyglucose Inhibitory postsynaptic potential Biochemistry chemistry.chemical_compound Methylamines Mice medicine Macrophage Animals Secretion Trypsin Molecular Biology Incubation Cells Cultured Chelating Agents Mice Inbred BALB C Methylamine Macrophages Zymosan Cell Biology beta-Galactosidase Galactosidases Phenylmethylsulfonyl Fluoride chemistry Lysosomes medicine.drug Research Article |
| Popis: | By utilizing compounds with different inhibitory properties, discrete biochemical differences were found in the mechanism of selective lysosomal enzyme secretion by macrophages in response to stimulation with zymosan particles and methylamine. Pretreatment of macrophages with trypsin markedly impaired the capacity of the cells to respond to stimulation with zymosan particles, but had no effect on methylamine-stimulated lysosomal enzyme secretion. Similarly, the addition of phenylmethanesulphonyl fluoride or EDTA to the incubation medium substantially inhibited zymosan-induced lysosomal enzyme secretion, whereas the methylamine-stimulated response was unaffected by these agents. The addition of 2-deoxyglucose to incubation media, however, strongly inhibited both zymosan- and methylamine-stimulated β-galactosidase secretion. These findings are consistent with a mechanism for lysosomal enzyme secretion by macrophages, based on a receptor-dependent uptake of zymosan particles and a receptor-independent uptake of methylamine. |
| Databáze: | OpenAIRE |
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