Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus)

Autor: Safaa I. Khater, Hemmat M. Eissa, Ahmed N. F. Neamat-Allah, Shaimaa A. Abd El-Kader, M.M. Fawzey, Sabah Ibrahim Khater, Doaa I.A. Mostafa, Doaa Ibrahim, Seham M. Ibrahim
Rok vydání: 2020
Předmět:
0301 basic medicine
Fish Proteins
Antioxidant
medicine.medical_treatment
Aquatic Science
Biology
Antioxidants
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Nile tilapia
Fish Diseases
Random Allocation
Selenium
Cytochrome P-450 Enzyme System
medicine
Environmental Chemistry
Animals
Food science
Heat-Shock Proteins
Disease Resistance
chemistry.chemical_classification
Chitosan
Dose-Response Relationship
Drug
Glutathione peroxidase
Caspase 1
04 agricultural and veterinary sciences
General Medicine
Cichlids
Malondialdehyde
biology.organism_classification
Animal Feed
Immunity
Innate
Aeromonas hydrophila
Diet
Oreochromis
030104 developmental biology
chemistry
Catalase
Dietary Supplements
040102 fisheries
biology.protein
0401 agriculture
forestry
and fisheries
Nanoparticles
Gram-Negative Bacterial Infections
Transcriptome
Zdroj: Fishshellfish immunology. 110
ISSN: 1095-9947
Popis: Nowadays there is a great attention for nanotechnology in aquaculture production. It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction. Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia. Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus. A total of 400 fish were randomly divided into four groups, and each group retained five replicates. The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet. The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively. Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization. Additionally, the expression of myostatin gene was down-regulated by 0.2 and 0.3 fold in group fed 0.5 and 1 g/kg SeChNPs when compared with control group. Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level. Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group. In contrast, the levels of C- reactive protein and malondialdehyde were reduced. The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner. Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%). After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs. Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group). In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
Databáze: OpenAIRE
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