Detection of antibodies to Anisakis simplex larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens
| Autor: | R. Gutiérrez Ramos, M. Tsuji |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Antibodies
Helminth Enzyme-Linked Immunosorbent Assay Immunoelectrophoresis Anisakiasis Anisakis Serology Antigen medicine Parasite hosting Animals Humans chemistry.chemical_classification biology medicine.diagnostic_test Anisakis simplex General Medicine biology.organism_classification Molecular biology Enzyme chemistry Antigens Helminth Larva biology.protein Chromatography Gel Animal Science and Zoology Parasitology Rabbits Antibody |
| Zdroj: | Journal of helminthology. 68(4) |
| ISSN: | 0022-149X |
| Popis: | The enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were used for the serodiagnosis of larvalAnisakis simplexinfections in man and immunized rabbits. Sephacryl S-300 gel filtration was used for separating crude antigen. Four fractions were obtained. Sera from patients with other helminth infections sometimes cross reacted withAnisakislarval antigens. With IEP, crude antigen is more sensitive than purified antigens. With ELISA, the third fraction is the most sensitive for detecting antibodies toAnisakislarvae in the sera of humans and immunized rabbits. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|